Anti-IFT81 Rabbit Polyclonal Antibody for WB, ELISA - Q8WYA0

Core Product Specifications and Parameters

Biological Background: IFT81 Function and Localization

• IFT81 (also known as CDV-1, DV1, CDV1R, or SRTD19) is a key subunit of the intraflagellar transport (IFT) complex B. Related references: PMID:23990561
• Within the IFT-B complex, IFT81 and IFT74 constitute a tubulin-binding module; the calponin-homology (CH)-like region of IFT81 directly engages tubulin to facilitate its ciliary transport. Related references: PMID:23990561
• IFT81 is indispensable for ciliogenesis; its ablation impairs cilium formation. Related references: PMID:23990561, PMID:27666822
• The protein is necessary for proper regulation of Sonic Hedgehog (SHH) signaling, a pathway that depends on functional primary cilia. Related references: PMID:27666822
• Expression of IFT81 is tissue-specific: high in testis, moderate in ovary, heart, liver, skeletal muscle, kidney, and pancreas; the testis-abundant isoform CDV-1R plays a role in spermatogenesis (by similarity).
• Subcellular localization is predominantly at the cilium, basal body, and cytoplasm, reflecting its trafficking function.
• Annotated keywords include acetylation, phosphoprotein, ciliopathy, differentiation, and spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 550–676 (C-terminal region) of human IFT81; the antibody may preferentially recognize epitopes in this region. For Western blot, a band at approximately 80 kDa is expected in whole-cell lysates of human, mouse, or rat origin.
• Cross-reactivity with mouse and rat has been reported; however, sequence conservation suggests possible reactivity with other species—validate in your sample system.
• For ELISA, consider using the recombinant protein fragment as a coating antigen; titration of antibody and antigen is recommended to establish optimal signal-to-noise ratio.
• Given IFT81's role in cilia, testis, and SHH signaling, inclusion of appropriate positive controls (e.g., ciliated cell lines, testis tissue) and negative controls (e.g., IFT81 knockout/knockdown samples) is advisable to confirm specificity.
• Avoid freeze-thaw cycles; aliquot and store at –20°C to –80°C upon receipt. Shipment on ice bags maintains stability during transit.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting your journey from concept to clinic. This anti-IFT81 rabbit polyclonal antibody is part of our extensive catalog for ciliary research, SHH pathway analysis, and spermatogenesis studies. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary supplies, and can assist with custom sourcing for rare targets to accelerate your assay development.