Anti-IFT74 Polyclonal Antibody for WB, ELISA - Q96LB3

Core Product Specifications and Parameters

Biological Background: IFT74 Function and Localization

• IFT74, also known as CMG-1, CCDC2, or BBS22, is a component of the intraflagellar transport (IFT) complex B and contains a coiled-coil domain.
• Together with IFT81, IFT74 forms a tubulin-binding module that mediates transport of tubulin within the cilium. Related references: PMID:23990561
• It is required for ciliogenesis and is essential for flagellogenesis during spermatogenesis. Related references: PMID:23990561 PMID:33689014
• Subcellular localization includes the cilium, flagellum, cytoplasmic vesicles, and the acrosome, reflecting its role in ciliary and flagellar transport.
• Tissue specificity: highly expressed in adult and fetal kidney, with lower expression in heart, placenta, lung, liver, and pancreas; detected in sperm at the protein level. Related references: PMID:11683410 PMID:33689014
• Keywords: alternative splicing, Bardet-Biedl syndrome, ciliopathy, Joubert syndrome; IFT74 undergoes methylation and phosphorylation, implicating post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1–372, targeting the N-terminal region of IFT74; the antibody may recognize full-length protein and N-terminal fragments.
• For Western blot, a major band near 69 kDa is expected under reducing conditions; confirm with appropriate positive controls (e.g., ciliated cell lysates).
• Cross-reactivity with mouse and rat is indicated; epitope conservation should be verified in your specific experimental model.
• For ELISA applications, consider direct antigen coating or use in a sandwich format with a validated pair; optimize coating conditions and antibody dilution.
• As a polyclonal antibody, it may offer broad epitope coverage; screen multiple clones if a monoclonal format is later required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, from antibodies to ancillary reagents, covering every stage from concept to clinic. This anti-IFT74 antibody, specific for ciliopathy and spermatogenesis targets, exemplifies our commitment to supplying optimized reagents for demanding applications. Whether you need validated antibody pairs, bulk materials, or assistance with rare target sourcing, CamelBio is your go-to partner for accelerated assay development.