Anti-IFT57 Rabbit Polyclonal Antibody for WB, ELISA - Q9NWB7

Core Product Specifications and Parameters

Biological Background: IFT57 Function and Localization

• Essential for the formation of cilia; defects are linked to ciliopathies.
• Plays an indirect role in sonic hedgehog signaling, as cilia are required for hedgehog pathway activity.
• Interacts with HIP1 to recruit caspase-8 (CASP8) and trigger apoptosis.
• May act as a transcription regulator by binding the DNA sequence motif 5'-AAAGACATG-3' present in the promoters of caspase genes.
• Together with RAB23 and KIF17, required for localization of specific G protein-coupled receptors, such as dopamine receptor DRD1, to primary cilia. Related references: PMID:26182404
• Localizes to cell projections, specifically the cilium, as well as the cytoplasm and cilium basal body.
• Expressed in many tissues including brain, thymus, lymph node, lung, liver, skin, and kidney (at protein level).

Experimental Guidance and Technical Tips

• For western blotting, consider using whole-cell lysates from human, mouse, or rat cell lines known to express IFT57.
• The immunogen covers amino acids 170–429, encompassing the coiled-coil domain; confirm specificity with appropriate controls.
• As a polyclonal antibody, validate performance in your specific sample type and application to account for potential lot variability.
• For cilia research, employ cilium-enrichment protocols to maximize detection sensitivity.
• In ELISA, optimize the coating antigen concentration and antibody dilution to achieve the desired signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and researchers with a complete range of IVD raw materials and technical support, from initial development to clinical application. For studies on primary cilia, hedgehog signaling, or ciliopathy mechanisms, we supply validated antibodies like this anti-IFT57 polyclonal, along with ancillary reagents and bulk options. Our sourcing capabilities extend to rare targets and customized antibody development, ensuring you have the materials needed to advance your work.