Anti-IFRD1 Polyclonal Antibody for WB, ELISA - O00458

Core Product Specifications and Parameters

Biological Background: IFRD1 Function and Localization

• IFRD1 (Interferon-related developmental regulator 1) is also known as nerve growth factor-inducible protein PC4 and TIS7, encoded by alternative splicing variants.
• The protein is implicated in the regulation of gene activity downstream of NGF signaling, potentially influencing both proliferative and differentiative cellular pathways.
• IFRD1 may act as an autocrine factor that either attenuates or amplifies the initial ligand-induced signal, fine-tuning the cellular response.
• Its expression is detected across a variety of tissues, consistent with a broad developmental role.
• Keywords associated with IFRD1 include alternative splicing, developmental protein, and differentiation, reflecting its involvement in cellular fate decisions.
• The calculated molecular mass of the canonical isoform is approximately 50 kDa, consistent with the observed band size.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (aa 1‑240) of human IFRD1. This design may favor detection of full‑length and possibly some truncated variants.
• For Western blot, consider loading 20‑30 μg of total protein lysate and using a standard ECL detection system; validate band specificity using siRNA knockdown or appropriate knockout controls.
• ELISA usage may require optimization of coating antigen and antibody dilution; serial dilutions are recommended to establish an optimal working concentration.
• As the antibody cross-reacts with mouse and rat, it is suitable for comparative studies; confirm performance in the relevant sample system by pilot experiments.
• Because IFRD1 expression is NGF‑inducible, timing of NGF treatment in cell models should be carefully controlled to capture dynamic changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to provide validated raw materials and tailored sourcing solutions for IVD development. Our portfolio includes monoclonal and polyclonal antibodies, antigen-grade recombinant proteins, and ancillary reagents — all designed to accelerate your assay pipeline from biomarker discovery to regulatory submission. For niche targets like IFRD1, we leverage deep biological expertise and a global supplier network to secure high-quality, cost‑effective raw materials, so you can focus on delivering reproducible results.