Anti-IFNL3 Polyclonal Antibody for WB & ELISA - Q8IZI9

Core Product Specifications and Parameters

Biological Background: IFNL3 Function and Localization

• IFNL3 (interferon lambda-3), also known as IL-28B/IL-28C, is a cytokine belonging to the type III interferon family.
• It plays a critical role in antiviral host defense, predominantly in epithelial tissues, by binding to the heterodimeric receptor IL10RB/IFNLR1 and activating the JAK/STAT signaling pathway to induce interferon-stimulated genes (ISGs).
• The receptor IFNLR1 has epithelial cell-specific expression, resulting in restricted targets and cell-type selective action of IFNL3.
• IFNL3 is involved in Toll-like receptor (TLR)-induced antiviral defense and contributes to the antiviral immune response in the intestinal epithelium.
• It also exerts immunomodulatory effects, including up-regulation of MHC class I antigen expression.
• The protein is secreted and contains a signal peptide and disulfide bonds, with a molecular weight of approximately 21.7 kDa.
• Alternative names include cytokine Zcyto22, interleukin-28B, and interleukin-28C.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human IFNL3. This peptide sequence may influence antibody specificity; validate in the relevant sample system.
• For Western blot, an expected band at ~22 kDa can be observed; consider using human or mouse epithelial cell lysates.
• Given the polyclonal nature and cross-reactivity with mouse, careful titration and inclusion of appropriate controls (e.g., peptide blocking) are recommended to confirm specificity.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a monoclonal antibody recognizing a distinct epitope could enhance assay performance.
• Always optimize concentrations and conditions in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of IVD raw materials, including validated antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This IFNL3 polyclonal antibody supports antiviral and immunomodulatory research, and we offer further support through matched antibody pairs, recombinant proteins, and tailored procurement solutions to accelerate your workflow from concept to clinic.