Anti-IFNL3 Polyclonal Antibody for IHC-P and ELISA - Q8IZI9

Core Product Specifications and Parameters

Biological Background: IFNL3 Function and Localization

• Interferon lambda-3 (IFNL3), also known as IL-28B/IL-28C, is a member of the type III interferon family and acts as a cytokine with antiviral, antitumour, and immunomodulatory activities.
• IFNL3 signals through a heterodimeric receptor composed of IL10RB and IFNLR1, leading to JAK/STAT pathway activation and induction of interferon-stimulated genes (ISGs) that establish an antiviral state.
• Due to the restricted expression of its receptor IFNLR1 primarily in epithelial cells, IFNL3 exerts its effects predominantly in epithelial tissues, playing a crucial role in mucosal antiviral defense, particularly in the intestinal epithelium.
• IFNL3 contributes to Toll-like receptor (TLR)-induced antiviral responses and enhances immune surveillance by up-regulating MHC class I antigen expression.
• The protein is secreted and contains a signal peptide; its mature form has a predicted molecular weight of approximately 21.7 kDa.
• Keywords associated with IFNL3 include antiviral defense, cytokine, disulfide bond, and secreted, highlighting its role in extracellular immune signaling.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised in rabbit against a recombinant fragment (aa 22-196), it may recognize multiple epitopes; consider optimizing dilution and blocking conditions for each application.
• For IHC on paraffin-embedded sections (IHC-P), antigen retrieval steps may be required, and validation in appropriate epithelial tissues is recommended given IFNL3's expression profile.
• In ELISA applications, the antibody can be employed as a detection or capture reagent; for sandwich ELISA, pairing with a monoclonal antibody against a non-overlapping epitope may enhance specificity.
• The reported cross-reactivity with mouse and rat IFNL3 should be verified using species-specific positive and negative controls in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For research on interferon lambda-3 and epithelial antiviral immunity, this anti-IFNL3 rabbit pAb is a valuable tool; we also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare targets to support your assay development and production needs.