Anti-IFNGR2 Polyclonal Antibody for WB and ELISA - P38484

Core Product Specifications and Parameters

Biological Background: Interferon gamma receptor 2 Function and Localization

• IFNGR2 encodes the interferon gamma receptor 2 protein, also known as AF-1, IFN-gamma receptor beta-chain, or interferon gamma transducer 1, which is a single-pass type I membrane protein.
• It associates with IFNGR1 to form a functional receptor for interferon gamma (IFNG), a critical cytokine in immune responses. Related references: PMID:7615558 PMID:7673114 PMID:8124716
• Upon IFNG binding, the receptor activates the JAK/STAT signaling pathway, leading to downstream transcriptional regulation of immune-related genes. Related references: PMID:15356148 PMID:7673114 PMID:8124716
• The protein is localized at the cell membrane and also found in cytoplasmic vesicles, Golgi apparatus membrane, and endoplasmic reticulum membrane, reflecting its trafficking and signaling compartments.
• IFNGR2 is expressed in T-cells, consistent with its role in adaptive immunity.
• Post-translational modifications include glycosylation and disulfide bond formation, and it contains a signal peptide, transmembrane domain, and cytoplasmic region.
• Structurally, the protein has a molecular weight of approximately 38 kDa (UniProt: 37806 Da) and features a conserved interferon-gamma receptor domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 238-337 of human IFNGR2, a region that encompasses part of the cytoplasmic domain; thus, the antibody may be suitable for detecting both membrane-bound and internalized receptor pools.
• For Western blotting, consider using reduced or non-reduced conditions based on experimental needs; the predicted molecular weight is ~38 kDa.
• When performing ELISA, ensure the coating antigen presents the immunogenic sequence; use appropriate positive controls (e.g., IFNGR2-expressing cells or recombinant protein).
• Validate cross-reactivity in mouse and rat samples, as homology in this region may vary.
• As a polyclonal antibody, it may exhibit slight lot-to-lot variability; revalidate each new lot under the specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services. This anti-IFNGR2 polyclonal antibody supports your interferon signaling research, and we complement it with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your procurement from concept to clinic.