Anti-IFNGR1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P15260

Core Product Specifications and Parameters

Biological Background: IFNGR1 Function and Localization

• IFNGR1 (Interferon gamma receptor 1) encodes the alpha subunit of the interferon gamma receptor, playing a pivotal role in antimicrobial, antiviral, and antitumor immune responses by activating effector cells and enhancing antigen presentation (PubMed:20015550). Related references: PMID:20015550
• The functional receptor is a heterodimer of IFNGR1 and IFNGR2; upon IFN-gamma binding, the intracellular domain of IFNGR1 engages JAK1 and JAK2, leading to STAT1 phosphorylation, dimerization, nuclear translocation, and transcription of immune effector genes (PubMed:10986460, PubMed:2971451, PubMed:7615558, PubMed:28883123). Related references: PMID:10986460, PMID:2971451, PMID:7615558, PMID:28883123
• Phosphorylated IFNGR1 provides a docking site for SOCS1, which negatively regulates JAK-STAT signaling by competing with STAT1 binding (By similarity).
• The protein is expressed on the cell membrane and features a signal peptide, an extracellular immunoglobulin-like domain, a single-pass transmembrane helix, and a cytoplasmic tail required for signal transduction.
• Post-translational modifications include disulfide bond formation, glycosylation, phosphorylation, and ubiquitination, which modulate receptor stability, signaling, and turnover.
• Alternative splicing generates multiple isoforms, and mutations in IFNGR1 are associated with Mendelian susceptibility to mycobacterial disease (IMD27A, IMD27B).
• It belongs to the type II cytokine receptor family and is a component of the reference proteome, highlighting its fundamental role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the C-terminal cytoplasmic region (aa 390-489), suggesting that the antibody should detect full-length IFNGR1 under denaturing conditions (e.g., Western blot) and may recognize the native protein in immunofluorescence/immunocytochemistry.
• For ELISA applications, consider pairing with a detection antibody that binds a distinct epitope to avoid competition, and validate with recombinant IFNGR1 or IFN-gamma stimulated cell lysates.
• Confirm cross-reactivity in mouse and rat samples by aligning the immunogen sequence with the corresponding region in these species; optimal dilutions should be determined empirically.
• Include appropriate controls such as IFNGR1 knockout or knockdown cell lines, and verify signal specificity by observing the expected molecular weight (~54 kDa) and blocking with the immunogen peptide if needed.

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