Anti-IFNGR1 Monoclonal Antibody for WB, ELISA - P15260

Core Product Specifications and Parameters

Biological Background: IFNGR1 Function and Localization

• Interferon gamma receptor 1 (IFNGR1), also known as CD119 or IFN-gamma-R-alpha, is a single-pass type I membrane protein and a critical subunit of the interferon gamma receptor complex.
• Serves as the ligand-binding receptor for interferon gamma (IFNG), playing a key role in antimicrobial, antiviral, and antitumor immune responses by activating effector cells and enhancing antigen presentation. Related references: PMID:20015550
• Associates with the accessory factor IFNGR2 to form a functional receptor complex upon ligand binding. Related references: PMID:10986460, PMID:2971451, PMID:7615558
• Upon IFNGR1 engagement, the intracellular domain undergoes conformational changes allowing association of JAK1 and JAK2 kinases; activated JAK1 phosphorylates IFNGR1 to create a docking site for STAT1, leading to STAT1 phosphorylation, dimerization, nuclear translocation, and target gene transcription. Related references: PMID:28883123
• STAT3 can also be activated through IFNGR1, though the response is typically weaker.
• The intracellular domain phosphorylation also provides a docking site for SOCS1, which negatively regulates JAK-STAT signaling by competing with STAT1 binding (By similarity).
• Expressed on the cell membrane.
• Keywords: Glycoprotein, disulfide bond, alternative splicing, disease variant, phosphoprotein, and Ubl conjugation, highlighting its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the intracellular region (aa 270–489), suggesting the antibody may detect total IFNGR1 protein by Western blot regardless of ligand binding or membrane localization.
• For Western blot, a band around 54 kDa is expected; consider using reducing conditions and appropriate controls (e.g., IFNGR1‑knockout validated cell lysates) to confirm specificity.
• The antibody is recommended for ELISA; ensure appropriate coating buffers and detection systems are optimized for the target protein.
• Cross‑reactivity with mouse and rat IFNGR1 has been reported; verify performance in the relevant species and tissue types before quantitative analyses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive one-stop platform for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This IFNGR1 antibody supports studies in immunology, infectious disease, and therapeutic monitoring, and our team can assist with sourcing complementary reagents such as IFNGR2 antibodies or recombinant proteins. As a dedicated B2B partner, we streamline your procurement from concept to clinic.