Anti-IFNAR1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P17181

Core Product Specifications and Parameters

Biological Background: IFNAR1 Function and Localization

• IFNAR1 (Interferon alpha/beta receptor 1), also known as Cytokine receptor class-II member 1 (CRF2-1), forms a heterodimeric receptor with IFNAR2 for type I interferons (IFN-α, IFN-β, IFN-ε, IFN-ω, and IFN-κ). Related references: PMID:10049744, PMID:14532120, PMID:15337770

• Upon interferon binding, IFNAR1 and IFNAR2 proximity triggers cross-phosphorylation of associated Janus kinases (TYK2 on IFNAR1, JAK1 on IFNAR2), phosphorylation of receptor tyrosine residues, recruitment and phosphorylation of STAT transcription factors, and their nuclear translocation to regulate interferon-stimulated gene expression. Related references: PMID:21854986, PMID:32972995, PMID:7665574, PMID:7526154, PMID:19561067

• IFNAR1 can also act independently of IFNAR2 as a functional receptor for IFNB1, activating a non-JAK-STAT signaling pathway.

• Subcellular localization includes the cell membrane, late endosomes, and lysosomes, consistent with receptor internalization and trafficking.

• IFN receptors are ubiquitously expressed. Multiple isoforms exist: isoform 1, 2, and 3 are detected in IFN-sensitive myeloma U266B1 cells, while only isoforms 2 and 3 are found in IFN-resistant U266R cells.

• IFNAR1 is a member of the class II cytokine receptor family, possessing extracellular fibronectin type III repeats and a single transmembrane helix.

• Key post-translational modifications include glycosylation, palmitoylation, phosphorylation, and ubiquitination, along with disulfide bond and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (aa 470–557), making the antibody suitable for applications like Western blotting (WB) and immunohistochemistry (IHC) that detect total IFNAR1 regardless of extracellular domain shedding or glycosylation state.
• For immunofluorescence (IF/ICC), permeabilization is necessary to allow antibody access to the intracellular epitope; include appropriate negative controls to verify specificity.
• As a polyclonal antibody, it may recognize multiple epitopes, enhancing sensitivity but potentially increasing background; consider optimizing blocking and washing conditions.
• Confirmed cross-reactivity with mouse and rat IFNAR1, but sequence homology in the immunogen region should be verified; validation in the relevant model system is recommended.
• For ELISA applications, ensure that the coating antigen matches the immunogen region or full-length protein; a sandwich ELISA format may improve signal-to-noise ratio.
• Keep cell/tissue lysates and reagents on ice during preparation to minimize proteolytic degradation of the ~64 kDa target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, core laboratories, and research institutes with comprehensive IVD raw material solutions, technical support, and consultative expertise from concept to clinic. As your partner for interferon signaling studies, we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare target raw materials tailored to your assay development pipeline. Reach out to discuss how we can streamline your procurement for IFNAR1 and other immune receptor targets.