Anti-IFN gamma Rabbit Polyclonal Antibody for WB, ELISA - P01579

Core Product Specifications and Parameters

Biological Background: IFN gamma Function and Localization

• Interferon gamma (IFN gamma) is a type II interferon, also known as immune interferon, encoded by the IFNG gene in humans. It is secreted primarily by activated T lymphocytes and natural killer cells.
• Functions as a potent cytokine in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093 PMID:8666937
• Signals through the JAK-STAT pathway upon binding to its receptor IFNGR1, leading to STAT1 activation and transcription of IFN gamma-regulated genes such as IRF1. Related references: PMID:8349687 PMID:16914093
• Enhances MHC class I antigen presentation by inducing immunoproteasome subunits and the activator PA28, increasing the quantity and quality of peptides for MHC loading. Related references: PMID:8666937 PMID:8163024 PMID:11112687
• Up-regulates MHC class II complex expression by promoting key molecules such as cathepsins B, H, and L. Related references: PMID:7729559
• Involved in the regulation of hematopoietic stem cells, affecting their development, quiescence, and differentiation during homeostasis (By similarity).
• Subcellular localization is secreted, consistent with its role as a cytokine. The pro-protein contains a signal peptide and undergoes cleavage on a pair of basic residues to generate the mature form.
• Post-translational modifications include glycosylation and pyroglutamic acid formation, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans the mature IFN gamma sequence (aa 24-166), targeting the functional domain; consider this when designing blocking peptides or competition assays.
• For Western blot, the predicted molecular weight is 19 kDa; ensure appropriate gel percentage and transfer conditions for low molecular weight proteins.
• Since IFN gamma is a secreted cytokine, sample preparation should consider its presence in serum, plasma, or cell culture supernatants; include appropriate controls and protease inhibitors to prevent degradation.
• For ELISA, this polyclonal antibody can be used as either capture or detection antibody; however, pairing with a monoclonal antibody for sandwich ELISA may improve specificity.
• Validate performance in relevant biological samples (e.g., activated T-cell lysates or stimulated PBMCs) to confirm reactivity and to establish optimal working dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers this IFN gamma polyclonal antibody alongside a comprehensive portfolio of diagnostic-grade reagents, including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. With technical services and consulting from concept to clinic, we support your entire assay development workflow, ensuring reliable supply for your IVD manufacturing needs.