Anti-IFN-gamma Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P01579

Core Product Specifications and Parameters

Biological Background: IFN-gamma Function and Localization

• Interferon gamma (IFN-gamma, IFG) is a type II interferon and cytokine, also known as immune interferon, produced by immune cells such as T-cells and NK cells.
• It plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Related references: PMID:16914093, PMID:8666937
• IFN-gamma signals primarily through the JAK-STAT pathway after binding to its receptor IFNGR1, leading to STAT1 activation, nuclear translocation, and transcription of target genes. Related references: PMID:8349687, PMID:16914093
• It enhances the class I antigen presentation pathway by inducing immunoproteasome subunits and the proteasome activator PA28, thereby increasing the quantity and quality of peptides for MHC class I loading. Related references: PMID:8666937, PMID:8163024, PMID:11112687
• IFN-gamma also up-regulates MHC class II expression by promoting cathepsins B, H, and L, contributing to efficient antigen processing. Related references: PMID:7729559
• The protein is secreted and plays a role in regulating hematopoietic stem cells during development and homeostasis.
• IFN-gamma is a glycoprotein with antiviral defense and growth regulation properties, and is of pharmaceutical interest.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the mature IFN-gamma sequence, likely recognizing native conformation; consider this when designing assays.
• For western blot (WB), use whole cell lysates from activated human T-lymphocytes or recombinant IFN-gamma as positive controls.
• In immunoprecipitation (IP) and ELISA, evaluate suitable antibody pairings and blocking conditions to avoid non-specific binding.
• For immunostaining applications (IF/ICC, IHC-P), optimize fixation/permeabilization and antigen retrieval protocols in the relevant tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing of IVD raw materials, combining validated antibody reagents, bulk ancillary supplies, and expert technical consulting to accelerate diagnostic development from concept to clinic. This IFN-gamma rabbit monoclonal antibody supports research into immune function and infectious diseases. From assay-matched monoclonal antibodies to rare target raw materials, CamelBio delivers tailored procurement solutions for your diagnostic pipeline.