Anti-IFN-beta Rabbit Monoclonal Antibody for WB, ELISA - P01574

Core Product Specifications and Parameters

Biological Background: IFN-beta Function and Localization

• IFN-beta (also known as fibroblast interferon, IFB, IFF, IFNB) is encoded by the IFNB1 gene in humans.
• It is a type I interferon cytokine that plays a key role in the innate immune response to infections, developing tumors, and other inflammatory stimuli. Related references: PMID:10049744, PMID:10556041, PMID:6157094
• Signaling occurs through binding to the heterodimeric receptor IFNAR1/IFNAR2, activating the canonical JAK-STAT pathway to regulate transcription of interferon-regulated genes. Related references: PMID:10049744, PMID:10556041, PMID:7665574
• Beyond antiviral and antibacterial activities, IFN-beta regulates B-cell development, myelopoiesis, and LPS-inducible production of tumor necrosis factor.
• In the nervous system, IFN-beta promotes neuronal autophagy and clearance of alpha-synuclein, helping protect dopaminergic neurons.
• Compared to interferon-alpha, IFN-beta is more potent in inducing apoptotic and antiproliferative pathways required for tumor cell growth control.
• IFN-beta is a secreted glycoprotein containing disulfide bonds and phosphorylation sites, as indicated by the keywords: Secreted, Glycoprotein, Disulfide bond, Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-187 of mature human IFN-beta, so the antibody is expected to detect the secreted, mature form. Consider using reducing SDS-PAGE conditions for WB.
• For WB, validate the antibody in appropriate human cell lines or stimulated samples known to express IFN-beta (e.g., virus-infected fibroblasts).
• In ELISA, consider pairing with another anti-IFN-beta antibody or using recombinant IFN-beta as standard for quantitative detection.
• Optimal working dilutions for each application should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop IVD raw-material partner, CamelBio supplies this IFN-beta antibody along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for immunoassay development. Whether you are building interferon-signaling diagnostic kits or sourcing rare cytokine raw materials, our integrated technical services bridge from concept to clinic.