Anti-IFN-beta Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P01575

Core Product Specifications and Parameters

Biological Background: IFN-beta Function and Localization

• IFN-beta is a type I interferon cytokine, a 22 kDa secreted glycoprotein, encoded by the Ifnb1 gene (mouse) or IFNB1 (human), with classical antiviral and immunomodulatory roles.
• Key player in innate immunity against viral and bacterial pathogens, and in tumor immune surveillance (PubMed:10708458, PubMed:23872679). Related references: PMID:10708458 PMID:23872679
• Signals through the heterodimeric IFNAR1/IFNAR2 receptor complex, activating JAK-STAT pathway to induce interferon-stimulated genes; can also signal via IFNAR1 alone independently of JAK-STAT (PubMed:23872679). Related references: PMID:23872679
• Exhibits broad antiviral and antibacterial activities, regulates B-cell development, myelopoiesis, and LPS-induced TNF production (PubMed:10708458, PubMed:14597717). Related references: PMID:10708458 PMID:14597717
• Protects dopaminergic neurons by promoting autophagy and α-synuclein clearance, maintaining dopamine turnover (PubMed:26451483). Related references: PMID:26451483
• More potent than IFN-alpha in inducing apoptosis and antiproliferative pathways in tumor cells (PubMed:14597717). Related references: PMID:14597717
• Subcellular localization: secreted. Post-translational modifications include disulfide bond, glycosylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length mouse IFN-beta precursor (aa 1–182), likely recognizing both precursor and mature secreted forms. For WB, expect a band near 22 kDa; consider inducing IFN-beta expression with viral mimics (e.g., poly I:C) or LPS in appropriate cell lines.
• For IF/ICC, use cells known to produce IFN-beta upon stimulation; fix and permeabilize appropriately to detect the secreted protein. Co-stain with organelle markers to confirm localization.
• When using ELISA, pair with a matched detection antibody for optimal sensitivity; validate the assay with recombinant IFN-beta standards.
• Cross-reactivity with human is expected based on sequence homology; validate performance on human cell lines (e.g., HeLa stimulated with poly I:C) to confirm specificity.
• As a polyclonal antibody, lot-to-lot consistency may vary; optimize working dilutions for each application and include appropriate positive/negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with streamlined access to critical raw materials like this anti-IFN-beta antibody, essential for innate immunity and cytokine profiling studies. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for hard-to-find targets—backed by expert technical and logistical support to accelerate your IVD assay development.