Anti-IFI27 Rabbit Polyclonal Antibody for WB and ELISA - P40305

Core Product Specifications and Parameters

Biological Background: IFI27 Function and Localization

• IFI27, also known as ISG12A, is an interferon alpha-inducible protein encoded by the IFI27 gene. Alternative splicing produces multiple isoforms.
• Functions as an adapter protein in type-I interferon-induced and TNFSF10-induced apoptosis. Promotes cytochrome C release from mitochondria, activating BAX and caspases 2, 3, 6, 8, and 9. Related references: PMID:18330707 PMID:24970806 PMID:27673746
• Plays a role in innate antiviral immunity against hepatitis C virus (HCV) by promoting ubiquitin-mediated proteasomal degradation of the viral NS5A protein via SKP2, and induces virus-induced pyroptosis by activating CASP3 after TRIM21-mediated 'Lys-6'-linked ubiquitination. Related references: PMID:27194766 PMID:27777077 PMID:36426955
• May negatively regulate transcription by enhancing XPO1-mediated nuclear export of NR4A1, NR4A2, and NR4A3 nuclear receptors, potentially influencing vascular injury responses. Related references: PMID:22427340
• Localizes to the mitochondrial membrane, inner nuclear membrane, and endoplasmic reticulum membrane.
• Contains a transit peptide and transmembrane helix. Post-translational modifications include ubiquitination (isopeptide bond), essential for its antiviral and apoptotic functions.
• Keywords: Alternative splicing, Antiviral defense, Apoptosis, Endoplasmic reticulum, Host-virus interaction, Immunity, Innate immunity, Membrane, Mitochondrion, Nucleus, Transcription regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human IFI27. This region exhibits high sequence conservation across species, and cross-reactivity with rat has been demonstrated. For other species, perform in silico alignment to predict reactivity.
• For Western blot, a specific band at approximately 12 kDa is expected in IFI27-expressing samples. Include appropriate positive and negative controls to confirm specificity.
• In ELISA, the antibody can serve as either a capture or detection reagent. Optimize coating and detection conditions empirically.
• As a polyclonal antibody, lot-to-lot consistency may vary. It is advisable to validate each new lot using standardized protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, technical services, and consulting. This IFI27 polyclonal antibody supports research in apoptosis and antiviral innate immunity, and is part of our extensive catalog of optimized antibodies for diagnostic development. Beyond antibodies, we provide bulk ancillary reagents and assist with rare target sourcing to help accelerate your assay development from concept to clinic.