Anti-IFI16 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q16666

Core Product Specifications and Parameters

Biological Background: IFI16 Function and Localization

• IFI16, also known as gamma-interferon-inducible protein 16, is a member of the PYHIN protein family that functions as a double-stranded DNA sensor with preference for supercoiled and cruciform DNA structures.
• It plays a central role in innate immunity by recognizing viral dsDNA in the cytosol and nucleus, leading to the induction of IFN-beta via the STING/TMEM173 pathway and the formation of nuclear caspase-1-activating inflammasomes.
• IFI16 is involved in transcriptional regulation, potentially acting as a repressor, and modulates cell cycle progression by interacting with p53/TP53 and the retinoblastoma protein, thereby influencing cellular proliferation and senescence.
• It localizes to both the nucleus and cytoplasm, and its subcellular distribution is crucial for its DNA sensing and signaling functions.
• An isoform of IFI16 specifically inhibits the AIM2 inflammasome by binding cytoplasmic dsDNA to prevent its detection by AIM2 and blocking the AIM2-PYCARD/ASC interaction; this isoform also weakly induces type I interferon-beta via STING1. Related references: PMID:30104205
• It exhibits anti-inflammatory activity by inhibiting the AIM2 inflammasome, likely through direct association with AIM2, and is necessary for IRF3 signaling during herpes simplex virus 1 infection.
• Post-translational modifications of IFI16 include acetylation, phosphorylation, and ubiquitination, which may regulate its activity in response to cellular signals.
• Broad tissue expression is observed, with presence in peripheral blood leukocytes, fibroblasts, lymphoid cells, and epithelial tissues such as prostate, ovary, and breast.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (aa 1–300) containing the PYRIN domain; epitope conservation across isoforms should be verified when detecting multiple variants.
• For western blot, the predicted molecular weight is 88 kDa, but post-translational modifications or isoform variants may produce additional bands; validate with appropriate controls.
• In immunohistochemistry (paraffin), consider optimizing antigen retrieval methods for each tissue type, as epitope accessibility can vary.
• Cross-reactivity with mouse and rat is indicated, but sequence homology in the immunogen region should be checked for these species to confirm potential reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to support diagnostic development. The IFI16 antibody is positioned within innate immunity and cancer research areas, where consistent raw material supply is critical. We bridge the gap from concept to clinic by providing scalable, traceable, and consultative supply solutions tailored to your assay requirements.