Anti-IER5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q5VY09

Core Product Specifications and Parameters

Biological Background: IER5 Function and Localization

• Immediate early response gene 5 protein (IER5), also known as SBBI48, functions as a transcription factor that mediates both positive and negative regulation of gene expression. Related references: PMID:22132193, PMID:25355627
• It positively regulates several chaperone genes during the heat shock response in a HSF1-dependent manner, contributing to thermal stress resistance. Related references: PMID:25355627, PMID:25816751
• IER5 negatively regulates CDC25B expression by associating with its promoter, thereby influencing cell cycle progression. Related references: PMID:22132193
• The protein plays a role in the dephosphorylation of HSF1 and S6K by facilitating the action of protein phosphatase PP2A, modulating stress signaling pathways. Related references: PMID:25816751, PMID:26496226
• IER5 is involved in cell proliferation control, thermal stress resistance, and survival following ionizing radiation, participating in cell cycle checkpoint responses. Related references: PMID:22132193, PMID:25355627, PMID:26496226, PMID:19238419
• Subcellularly, IER5 is found in both the nucleus and cytoplasm, consistent with its transcriptional regulatory and signaling roles.
• Expression is noted in acute myeloid leukemia (AML) cells, suggesting potential relevance in hematopoietic malignancies.
• Keywords associated with IER5 include activator, repressor, stress response, and transcription regulation, reflecting its multifaceted cellular functions.

Experimental Guidance and Technical Tips

• The immunogen lies within residues 200-300 of human IER5, a region that may provide specific recognition of the full-length protein and potential isoforms. Consider verifying specificity using knockdown or knockout controls in your sample system.
• For WB, the predicted protein weight is 34 kDa; validate band size in your samples and consider loading controls.
• IHC-P and IF/ICC applications may require optimization of antigen retrieval and blocking due to nuclear/cytoplasmic localization.
• Cross-reactivity with mouse and rat suggests conserved epitope; however, verify detection in these species under your experimental conditions.
• ELISA may be used for quantifying IER5 levels; include appropriate standards and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement with a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For transcription and stress-response targets like IER5, we source rare raw materials suited to diagnostic assay development. Contact us to discuss your next project and benefit from integrated supply and technical consulting.