Anti-IER3 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P46695

Core Product Specifications and Parameters

Biological Background: IER3 Function and Localization

• Protein identity and alternative names: IER3 is a radiation-inducible immediate-early gene protein, also known as IEX-1, DIF-2, PRG1, and GLY96.
• Role in ERK signaling: Inhibits the dephosphorylation of ERK by the PP2A-PPP2R5C holoenzyme, and functions as an ERK downstream effector to mediate cell survival.
• Stress resistance pathway: As a component of the NUPR1/RELB/IER3 survival pathway, it contributes to remarkable resistance against cell stress, such as starvation or gemcitabine treatment, particularly in pancreatic ductal adenocarcinoma.
• Subcellular localization: Localized to the membrane; features a signal-anchor and a transmembrane helix, consistent with its membrane association.
• Post-translational modifications and structural features: Classified as a glycoprotein and phosphoprotein, undergoing proteomic identification as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-150 of human IER3, a region that may exhibit conservation; verify target detection in your specific sample system.
• For Western blotting, the predicted molecular weight is approximately 17 kDa; consider using a 12-15% SDS-PAGE gel and optimize transfer conditions accordingly.
• In IHC-P, antigen retrieval protocols may need optimization; positive controls such as human or mouse tissue sections are advisable to confirm specific staining.
• For ELISA, the antibody can be tested as a capture or detection reagent using recombinant IER3 protein or cell lysates; validate performance with appropriate standards.
• Cross-reactivity is reported for human and mouse; confirm reactivity with your species and tissue of interest before experimental use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including this anti-IER3 antibody for investigating ERK signaling, stress resistance, and cancer biology. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. We support your development workflow from concept to clinic, enabling reliable assay construction and biomarker research.