Anti-IDS Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P22304

Core Product Specifications and Parameters

Biological Background: Iduronate 2-sulfatase Function and Localization

• Encoded by the IDS gene, Iduronate 2-sulfatase (IDS; also known as idursulfase, ID2S) is a lysosomal hydrolase critical for the degradation of glycosaminoglycans dermatan sulfate and heparan sulfate.
• The enzyme functions as a monomer and requires calcium for activity; it undergoes post‑translational processing including signal peptide cleavage, glycosylation, and disulfide bond formation.
• Alternative splicing generates multiple transcripts, though functional significance may vary.
• Mutations in IDS cause mucopolysaccharidosis type II (Hunter syndrome), a severe lysosomal storage disorder, highlighting the enzyme's essential role in lysosomal homeostasis.
• IDS localizes predominantly in the lysosome lumen where it acts to cleave sulfate groups from sulfated sugars.
• Tissue expression is highest in liver, kidney, lung, and placenta, consistent with its role in systemic glycosaminoglycan turnover.
• Keywords from UniProt include hydrolase, glycoprotein, signal, zymogen, and disease variant, underscoring its biochemical and clinical significance.

Experimental Guidance and Technical Tips

• The immunogen covers residues 34–290 of human IDS (NP_000193.1), corresponding to the mature processed form; therefore the antibody is likely to recognize the native and denatured enzyme.
• For Western blot, consider a starting dilution range of 1:500–1:2000 and validate in relevant lysates (e.g., liver, placenta) or recombinant protein; the predicted molecular weight is ~62 kDa, but glycosylation may shift band mobility.
• In immunohistochemistry (IHC‑P), use paraffin sections with appropriate antigen retrieval; include positive (e.g., liver) and negative (e.g., IDS‑deficient) controls.
• For immunofluorescence (IF/ICC), ensure permeabilization of lysosomal compartments; co‑staining with a lysosomal marker is recommended to confirm localization.
• ELISA applications may employ the antibody as capture or detection reagent; optimize coating and detection conditions using recombinant IDS protein.
• As a polyclonal antibody, lot‑to‑lot consistency should be verified for critical quantitative assays; consider affinity purification if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw‑material sourcing, from concept to clinic. For researchers targeting iduronate 2-sulfatase and lysosomal storage disorders, we offer ready‑to‑ship validated antibodies, bulk ancillary reagents, and custom development of matched antibody pairs. Let our supply network accelerate your diagnostic assay development for rare disease markers.