Anti-IDO1 Rabbit PolymAb® for WB, IF/ICC, ELISA - P14902

Core Product Specifications and Parameters

Biological Background: IDO1 Function and Localization

• IDO1 (Indoleamine 2,3-dioxygenase 1), also known as IDO or INDO, is a heme-containing enzyme that catalyzes the first and rate-limiting step of tryptophan catabolism along the kynurenine pathway.
• It plays a crucial role in peripheral immune tolerance by depleting tryptophan, which inhibits T lymphocyte proliferation, and by producing tryptophan catabolites that promote T-cell apoptosis and regulatory T-cell differentiation. Related references: PMID:25691885
• IDO1 acts as a suppressor of anti-tumor immunity; its expression in tumors is associated with a worse clinical outcome. Related references: PMID:14502282 PMID:23103127 PMID:25157255 PMID:18418598
• The enzyme protects the fetus from maternal immune rejection by locally depleting tryptophan at the maternal-fetal interface. Related references: PMID:25691885
• IDO1 also limits the growth of intracellular pathogens by depriving them of tryptophan. Related references: PMID:25691885
• Subcellularly, IDO1 is located in the cytoplasm, specifically in the cytosol.
• It is expressed in mature dendritic cells in lymphoid organs, some epithelial cells, and endothelial cells of the placenta, and is highly inducible in most tissues, with overexpression in over 50% of tumors.
• Keywords: Dioxygenase, Heme, Iron, Metal-binding, Oxidoreductase, Tryptophan catabolism, Immunity.

Experimental Guidance and Technical Tips

• This antibody is recommended for Western blotting (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA applications. For WB, consider optimizing loading amounts based on the expected molecular weight (~45 kDa).
• Given the immunogen design covering the full-length protein (amino acids 1-403), this antibody may recognize both native and denatured forms of IDO1 in human samples.
• Since cross-reactivity is validated only for human, ensure validation of specificity in your relevant experimental system, especially when using non-human samples.
• Perform pilot experiments to optimize antibody dilutions and incubation conditions for your specific application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. This Anti-IDO1 Rabbit PolymAb® targets the immunology and oncology research areas, and our team can assist with rare target sourcing, custom antibody development, and technical consulting to streamline your diagnostic pipeline from concept to clinic.