Anti-IDO1 Polyclonal Antibody for WB, IF/ICC, ELISA - P14902

Core Product Specifications and Parameters

Biological Background: IDO1 Function and Localization

• IDO1 catalyzes the first and rate-limiting step of tryptophan catabolism along the kynurenine pathway. Related references: PMID:17671174 PMID:18026683
• It plays a critical role in peripheral immune tolerance by depleting tryptophan, which inhibits T lymphocyte division, and by accumulating tryptophan catabolites that induce T-cell apoptosis and differentiation of regulatory T-cells. Related references: PMID:25691885
• IDO1 acts as a suppressor of anti-tumor immunity, contributing to tumor immune escape. Related references: PMID:14502282 PMID:23103127 PMID:25157255
• It limits the growth of intracellular pathogens by depriving them of tryptophan. Related references: PMID:25691885
• The enzyme protects the fetus from maternal immune rejection during pregnancy. Related references: PMID:25691885
• IDO1 is primarily localized in the cytoplasm and cytosol.
• Tissue expression is observed in mature dendritic cells in lymphoid organs, some epithelial cells, endothelial cells of placenta, and is inducible in most tissues; it is overexpressed in over 50% of tumors, correlating with poor clinical outcome.
• IDO1 is a heme-containing dioxygenase that binds iron, belonging to the tryptophan catabolism pathway.

Experimental Guidance and Technical Tips

• For Western blot, an expected band around 45 kDa may be observed; consider loading appropriate positive controls or using lysates from IDO1-expressing tissues.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions as IDO1 is a cytosolic protein.
• Validate antibody performance in the relevant sample system, as IDO1 expression is often inducible and varies across tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials and technical services, supporting projects from concept to clinic. This IDO1 antibody is suitable for immune regulation, oncology, and tryptophan metabolism studies. From validated antibody pairs to bulk ancillary reagents and rare target sourcing, we help accelerate your development pipeline.