Anti-IDH3B Rabbit Polyclonal Antibody for WB, IF, ELISA - O43837

Core Product Specifications and Parameters

Biological Background: IDH3B Function and Localization

• IDH3B (isocitrate dehydrogenase [NAD] subunit beta, mitochondrial) is a structural subunit of the mitochondrial NAD-dependent isocitrate dehydrogenase complex, essential for the tricarboxylic acid (TCA) cycle.
• It facilitates the assembly and full enzymatic activity of the heterotetramer (2α:1β:1γ) that catalyzes the decarboxylation of isocitrate to α-ketoglutarate, a key step in energy metabolism.
• The α/β heterodimer (IDH3A/IDH3B) exhibits basal catalytic activity, but maximal activity requires the cooperative interaction of both α/β and α/γ (IDH3A/IDH3G) heterodimers within the intact tetramer.
• IDH3B is localized to the mitochondrion and contains a transit peptide that directs its import; it is subject to post-translational acetylation.
• Alternative splicing generates multiple isoforms, and mutations in IDH3B are linked to retinitis pigmentosa, a form of inherited retinal degeneration.
• The protein has a predicted molecular weight of approximately 42 kDa (42,184 Da), consistent with its identification in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 35–170) excludes the N-terminal mitochondrial transit peptide, suggesting the antibody likely recognizes the mature, processed form of IDH3B.
• For Western blot (WB), anticipate a band near 42 kDa in mitochondrial-enriched lysates; validate with appropriate controls and consider using fresh or ice-packed samples as per shipping conditions.
• Immunofluorescence (IF) or immunocytochemistry (ICC) should reveal a mitochondrial staining pattern; co-staining with mitochondrial markers can confirm specificity.
• In ELISA applications, titrate the antibody to optimize signal-to-noise ratios for antigen quantitation.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for comparative studies across species, but verify performance in the specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and life science researchers with streamlined access to a wide spectrum of IVD raw materials. This anti-IDH3B polyclonal antibody supports mitochondrial metabolism and TCA cycle research, complementing our offerings in validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need rare target sourcing or scale-up support, CamelBio bridges the gap from concept to clinic.