Anti-IDH3A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50213

Core Product Specifications and Parameters

Biological Background: IDH3A Function and Localization

• IDH3A encodes the alpha subunit of mitochondrial NAD-dependent isocitrate dehydrogenase, a key enzyme of the tricarboxylic acid (TCA) cycle.
• It catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate, utilizing NAD⁺ as a cofactor and requiring divalent metal ions (Mg²⁺/Mn²⁺) for activity.
• The enzyme functions as a heterotetramer: two alpha subunits combine with one beta (IDH3B) and one gamma (IDH3G) subunit to form the fully active complex; alpha/beta and alpha/gamma heterodimers exhibit basal activity.
• The protein localizes exclusively to the mitochondrial matrix, consistent with its role in cellular respiration.
• Post-translational modifications include acetylation and phosphorylation, and alternative splicing generates multiple isoforms.
• Mutations in IDH3A are linked to retinitis pigmentosa, highlighting the importance of TCA cycle integrity in retinal health.
• Alternative names include Isocitric dehydrogenase subunit alpha and NAD(+)-specific ICDH subunit alpha.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 28–366 of human IDH3A, covering a significant portion of the mature protein; the antibody is expected to detect both endogenous and recombinant IDH3A.
• For western blot (WB), a band around 40 kDa (predicted molecular weight 39.6 kDa) should be observed; include appropriate positive controls (e.g., HeLa, HEK293, mouse/rat tissue lysates).
• In immunohistochemistry (IHC‑P), optimize antigen retrieval conditions (e.g., citrate buffer, pH 6.0) and validate signal specificity with blocking peptides or knockout tissue.
• For immunofluorescence (IF/ICC), confirm mitochondrial staining pattern using a mitochondrial marker co‑stain; permeabilization conditions may require optimization.
• Cross‑reactivity with mouse and rat allows translational studies; independent validation in the relevant sample system is recommended.
• The antibody is also suitable for ELISA applications; pairing it with other IDH3A antibodies may enable sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with reliable access to IVD raw materials, including validated antibody pairs, polyclonal and monoclonal antibodies, and ancillary reagents. This anti‑IDH3A antibody, targeting a central TCA cycle enzyme, integrates seamlessly into studies on mitochondrial metabolism and related disorders. For bulk orders or custom sourcing of rare‑target reagents, CamelBio offers a streamlined procurement pathway from concept to clinical application.