Anti-IDH2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - P48735

Core Product Specifications and Parameters

Biological Background: IDH2 Function and Localization

• IDH2 functions as a mitochondrial NADP-dependent isocitrate dehydrogenase that plays a critical role in intermediary metabolism and energy production (PubMed:19228619, PubMed:22416140). Related references: PMID:19228619, PMID:22416140
• IDH2 may tightly associate or interact with the pyruvate dehydrogenase complex, integrating TCA cycle activity with pyruvate metabolism (PubMed:19228619, PubMed:22416140). Related references: PMID:19228619, PMID:22416140
• The IDH2 protein is localized to the mitochondrial matrix (SUBCELLULAR LOCATION: Mitochondrion).
• The IDH2 gene encodes multiple protein isoforms through alternative splicing (Keywords: Alternative splicing).
• Post-translational acetylation of IDH2 has been reported, which may modulate its activity (Keywords: Acetylation).
• Enzymatic activity of IDH2 depends on divalent metal cofactors such as Mg²⁺ or Mn²⁺ (Keywords: Magnesium, Manganese, Metal-binding).
• Mutations in IDH2 are associated with D-2-hydroxyglutaric aciduria type 2 (D2HGA2) and are frequently observed in various cancers, making it a significant disease variant (Keywords: Disease variant; Alias D2HGA2).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 193–452 of human IDH2 (NP_002159.2), which encompasses a large portion of the catalytic domain, suggesting that the antibody may recognize full-length native IDH2 in multiple species due to high sequence conservation.
• For Western blot (WB), a band around 51 kDa is expected; consider using mitochondrial-enriched lysates or IDH2-overexpressing cell lines as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial staining patterns should be anticipated; counterstaining with mitochondrial markers can aid confirmation.
• When using chromatin immunoprecipitation (ChIP) or immunoprecipitation (IP), validate the antibody's performance in the relevant cell or tissue system, as the epitope may be occluded in certain protein complexes.
• As the antibody detects IDH2 in human, mouse, and rat, it is suitable for cross-species studies; however, confirm reactivity in your specific model system due to potential epitope variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like IDH2, which is central to metabolism and cancer research, CamelBio offers tailored sourcing solutions for rare targets, supporting your diagnostic development from concept to clinic.