Anti-IDH1 Polyclonal Antibody for WB, IF/ICC, ELISA - O75874

Core Product Specifications and Parameters

Biological Background: IDH1 Function and Localization

• IDH1 encodes cytoplasmic isocitrate dehydrogenase [NADP] (IDPc), which catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate to 2-oxoglutarate, a reaction required by downstream enzymes such as phytanoyl-CoA dioxygenase. Related references: PMID:10521434 PMID:19935646
• The enzyme generates NADPH, a critical cofactor for numerous biosynthesis pathways and antioxidant defense, linking it to cellular anabolism and redox homeostasis.
• IDH1 localizes predominantly to the cytosol and peroxisomes, and may also act as a corneal epithelial crystallin to maintain corneal transparency (by similarity).
• It is a metal-dependent enzyme that requires magnesium or manganese for activity, and is subject to acetylation and phosphorylation as revealed by proteomic studies.
• As a key component of the tricarboxylic acid cycle and glyoxylate bypass, IDH1 is involved in central carbon metabolism, with somatic mutations commonly found in gliomas and acute myeloid leukemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 100–200, which is conserved across human, mouse, rat, and monkey; this supports the reported broad species cross-reactivity for western blotting (WB) and immunofluorescence (IF/ICC).
• For WB, a band around 47 kDa is expected; consider using reducing conditions and loading controls in lysates from IDH1-expressing cell lines (e.g., HeLa, HepG2).
• In IF/ICC, validate localization patterns with organelle markers to confirm cytosolic and peroxisomal staining, as noted in literature.
• ELISA applications may require optimization of coating antigen and antibody dilution; a direct or sandwich ELISA with recombinant IDH1 protein can be used as a positive control.
• Note that the antibody is polyclonal and may exhibit lot-to-lot variability; consistent performance should be verified in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, providing validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target procurement. This anti-IDH1 polyclonal antibody supports research and assay development in cancer metabolism, the TCA cycle, and redox biology. For customized solutions or technical consultation, our team bridges your concept-to-clinic journey with reliable supply chain and expertise.