Anti-IDE Rabbit Monoclonal Antibody [KD Validated] for WB, IHC-P, ELISA - P14735

Core Product Specifications and Parameters

Biological Background: Insulin-degrading enzyme Function and Localization

• IDE (Insulin-degrading enzyme), also known as Insulysin, Abeta-degrading protease, or Insulin protease, is a zinc-dependent metalloprotease encoded by the IDE gene.
• It plays a crucial role in degrading insulin, glucagon, and islet amyloid polypeptide (IAPP), thereby regulating blood glucose homeostasis and peptide hormone signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531). Related references: PMID:10684867, PMID:17051221, PMID:17613531
• IDE degrades amyloid-beta (Aβ) peptides derived from amyloid precursor protein (APP), contributing to the clearance of neurotoxic Aβ aggregates and potentially impacting Alzheimer's disease pathology (PubMed:26394692, PubMed:9830016). Related references: PMID:26394692, PMID:9830016
• It also degrades natriuretic peptides (ANP, BNP, CNP) and an aberrant frameshifted form of NPPA (fsNPPA) linked to familial atrial fibrillation, modulating cGMP signaling (PubMed:21098034). Related references: PMID:21098034
• Subcellularly, IDE localizes to the cytoplasm, cytosol, cell membrane, and is secreted, with expression detected in brain and cerebrospinal fluid.
• The membrane-associated isoform functions as an entry receptor for varicella-zoster virus (VZV), highlighting a role in host-virus interaction.
• IDE is characterized as an allosteric enzyme with ATP-binding and metal-binding properties, and undergoes alternative splicing.
• Additionally, IDE participates in antigen processing, generating the MAGEA3-derived antigenic peptide presented by MHC class I.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-250 of human IDE; therefore, this antibody may detect full-length IDE or N-terminal fragments. Validate the specificity in your sample system.
• For Western blot, a band of approximately 118 kDa is expected for human IDE. Use appropriate protein ladders and consider positive control lysates from brain tissue or cell lines expressing IDE.
• In immunohistochemistry (IHC-P), optimize antigen retrieval conditions and use appropriate tissue sections; IDE is expressed in brain and other tissues, so include relevant positive and negative controls.
• For ELISA applications, the antibody can be considered as a capture or detection reagent; titrate to determine optimal working concentrations.
• As a [KD Validated] antibody, it may be suitable for knockdown confirmation studies; employ appropriate siRNA/shRNA controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-IDE antibody alongside complementary reagents such as antibody pairs, optimized controls, and bulk ancillary supplies. Whether your research focuses on metabolic disorders, Alzheimer's disease, or viral entry mechanisms, CamelBio can source rare-target raw materials to accelerate your diagnostic development from concept to clinic. Contact us for tailored procurement solutions.