Anti-ID3 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q02535

Core Product Specifications and Parameters

Biological Background: ID3 Function and Localization

• ID3 (inhibitor of DNA binding 3) is a transcriptional regulator that lacks a basic DNA-binding domain. It negatively regulates basic helix-loop-helix (bHLH) transcription factors by forming heterodimers, thereby inhibiting their DNA binding and transcriptional activity.
• ID3 is implicated in a broad range of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.
• In myogenesis, ID3 inhibits skeletal muscle and cardiac myocyte differentiation while promoting the proliferation of muscle precursor cells. It also prevents the binding of E2A-containing protein complexes to the muscle creatine kinase E-box enhancer.
• ID3 regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.
• The protein localizes to the nucleus, consistent with its role as a transcriptional repressor.
• ID3 is abundantly expressed in lung, kidney, and adrenal gland, but not in adult brain.
• Alternative names include Class B basic helix-loop-helix protein 25 (bHLHb25), Helix-loop-helix protein HEIR-1, and Inhibitor of differentiation 3.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ID3 protein (amino acids 1–119), likely yielding antibodies that recognize native conformational epitopes. For IHC-P, consider antigen retrieval conditions to expose these epitopes effectively.
• Given the polyclonal nature, the antibody may detect multiple species (human, mouse, rat) as listed, but cross-reactivity should be validated in the relevant sample system.
• For IF/ICC, optimize fixation and permeabilization protocols to preserve nuclear localization of ID3. Use appropriate controls (e.g., ID3 knockout or overexpression) to confirm specificity.
• In ELISA, the antibody can be used for direct antigen detection or as part of a capture/detection pair; titer and working dilution should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and research labs with streamlined access to validated IVD raw materials, including optimized antibody pairs, bulk ancillary reagents, and support for rare-target sourcing. For your ID3-related projects—whether exploring transcriptional regulation, myogenesis, or circadian biology—we can supply matched antibodies, recombinant proteins, and technical consultation to help move your assay from concept to clinical application.