Anti-ICT1/MRP-L58 Rabbit Polyclonal Antibody for WB, ELISA - Q14197

Core Product Specifications and Parameters

Biological Background: ICT1/MRPL58 Function and Localization

• ICT1, also known as MRP-L58, DS-1, and immature colon carcinoma transcript 1 protein, is a mitochondrial large ribosomal subunit protein (UniProt Q14197).
• It functions as an essential peptidyl-tRNA hydrolase, acting as a codon-independent translation release factor that lacks stop codon specificity, directing translation termination and ribosome recycling in the mitochondrion.
  Related references: PMID:20186120 PMID:33878294
• Specifically catalyzes the hydrolysis of peptidyl-tRNAs that have been prematurely terminated, thus facilitating the recycling of stalled mitochondrial ribosomes.
  Related references: PMID:20186120 PMID:33878294
• Subcellular localization is restricted to the mitochondrion.
• Expression of ICT1 is down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells, suggesting a role in cellular proliferation or differentiation.
• Post-translational modification includes methylation, as detected by proteomic analyses.
• Keywords from UniProt include Hydrolase, Methylation, Mitochondrion, Protein biosynthesis, Ribonucleoprotein, and Ribosomal protein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 30–206 of human ICT1 (NP_001536.1), which corresponds to the mature protein after cleavage of the mitochondrial transit peptide. This region contains the conserved peptidyl-tRNA hydrolase domain.
• For Western blotting, a band at approximately 24 kDa is expected in human and mouse cell lysates. Mitochondrial fraction enrichment may improve detection sensitivity.
• For ELISA, consider using the immunogen as a reference standard for assay optimization.
• Cross-reactivity has been confirmed with human and mouse samples; validation in other model organisms is recommended if needed.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to titrate the antibody for each new application or batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for sourcing high-quality IVD raw materials and technical support for diagnostic assay development. This ICT1/MRPL58 antibody can be integrated into research workflows focused on mitochondrial translation and ribosome-associated quality control. Beyond antibodies, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target raw materials to streamline your path from concept to clinic.