Anti-ICOS/CD278 Monoclonal Antibody for WB and ELISA - Q9Y6W8

Core Product Specifications and Parameters

Biological Background: ICOS/CD278 Function and Localization

• ICOS (Inducible T-cell costimulator), also known as CD278 or AILIM, is a stimulatory receptor expressed on activated or antigen-experienced T-cells that plays an important role in the immune response. Related references: PMID:9930702

• Upon binding to its ligand ICOSL on antigen-presenting cells, ICOS delivers costimulatory signals that enhance T-cell proliferation, lymphokine secretion (e.g., IL-10), and B-cell antibody production. Related references: PMID:33033255

• ICOS is critical for the differentiation of T follicular regulatory cells during immune challenges such as viral infection. Related references: PMID:27135603

• Mechanistically, ICOS potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling, and independently activates PI3K signaling pathways. Related references: PMID:30523347

• ICOS prevents apoptosis of pre-activated T-cells and is essential for CD40-mediated immunoglobulin class switching in B-cells.

• Subcellularly, ICOS localizes to the cell membrane and can be secreted. It undergoes post-translational modifications including glycosylation and disulfide bond formation.

• Tissue expression is mainly in activated T-cells, with high levels in tonsillar germinal centers; lower levels in thymus, lung, lymph node, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100), likely accessible in both native and denatured conformations; consider reducing/denaturing conditions for WB with activated T-cell lysates.
• For ELISA, direct peptide-based or capture assays may be suitable; validate with recombinant ICOS or cell-based expression systems.
• Confirm cross-reactivity in mouse and rat samples due to sequence conservation; pilot experiments with species-matched positive controls are recommended.
• The relatively low molecular weight (23 kDa) may require careful gel electrophoresis and transfer optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. For ICOS/CD278, an immune checkpoint target in T-cell biology, we offer monoclonal and polyclonal antibody options tailored to immunoassay development. Our sourcing capabilities cover rare targets and customized reagent supply, helping to streamline your projects from concept to clinic.