Anti-ICMT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60725

Core Product Specifications and Parameters

Biological Background: ICMT Function and Localization

• ICMT, also known as isoprenylcysteine carboxylmethyltransferase, catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, a critical step in the processing of prenylated proteins such as Ras and other small GTPases.
• Belongs to the methyltransferase family and uses S-adenosyl-L-methionine as a methyl donor (UniProt KEYWORDS: Methyltransferase, S-adenosyl-L-methionine, Transferase).
• Localizes to the endoplasmic reticulum membrane, consistent with its role in modifying CAAX motif proteins after prenylation (SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; KEYWORDS: Endoplasmic reticulum, Membrane, Transmembrane helix).
• Ubiquitously expressed, with higher levels in cerebellum and putamen; abundant in Purkinje cells and pontine neurons (TISSUE SPECIFICITY).
• Alternative names include PCMT, PPMT, PCCMT, HSTE14, MST098, MSTP098, reflecting historical nomenclature.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 175-284; for IHC-P, consider optimizing antigen retrieval methods due to ER localization.
• For WB, the predicted molecular weight is 32 kDa; validate specificity in relevant sample systems and consider potential prenylation-related shifts.
• Tested cross-reactivity includes human, mouse, and rat; for other species, verify based on sequence homology.
• Use appropriate controls (e.g., knockout/knockdown) to confirm target specificity in ELISA and other applications.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material partner, CamelBio offers a comprehensive range of validated antibodies for protein modification research, including ICMT for prenylation and methylation studies. We provide bulk ancillary reagents, optimized antibody pairs, and rare target sourcing to support diagnostic development and research from concept to clinic.