Anti-ICK Polyclonal Antibody for WB, ELISA - Q9UPZ9

Core Product Specifications and Parameters

Biological Background: ICK Function and Localization

• ICK (serine/threonine-protein kinase ICK), also known as CILK1, hICK, LCK2, and MRK, is a member of the MAK-related kinase family essential for ciliogenesis and developmental signaling.
• It is required for primary cilium formation; knockout or mutation leads to defective ciliogenesis. Related references: PMID:24797473
• ICK phosphorylates the kinesin motor protein KIF3A, a key component of intraflagellar transport (IFT) required for ciliary assembly and function.
• The kinase controls ciliary length by modulating IFT speed in a cAMP- and mTORC1-dependent manner. Related references: PMID:24853502
• ICK localizes to multiple subcellular compartments including the nucleus, cytoplasm, cytosol, and specifically to the cilium and ciliary basal body, underscoring its role in ciliary dynamics.
• Tissue expression is broad, with highest levels in placenta and testis, and expression in heart, brain, pancreas, thymus, prostate, ovary, and intestinal tissues; it is absent from spleen and is frequently detected in cancer cell lines.
• The protein is subject to alternative splicing and is classified as an ATP-binding, magnesium-dependent kinase involved in developmental processes and associated with ciliopathies, craniosynostosis, ectodermal dysplasia, and epilepsy.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–270 of human ICK (NP_057597.2), encompassing the kinase domain; this region may contribute to broad species cross-reactivity (human, mouse, rat) as validated by the supplier.
• For Western blot: consider using reducing conditions and probing lysates from ciliated cell lines or tissues known to express ICK (e.g., testis, placenta, or cancer lines). An ~71 kDa band is expected.
• As a polyclonal antibody, lot-to-lot consistency should be verified; include positive and negative controls (e.g., ICK knockout or siRNA-treated samples) to confirm specificity in your experimental system.
• ELISA-based detection may require pairing with a suitable capture antibody or optimized coating conditions; pilot experiments are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, specializing in antibodies like this anti-ICK polyclonal antibody for ciliogenesis and kinase studies. Our portfolio includes validated antibody pairs, bulk ancillary reagents, and custom sourcing solutions to accelerate your assay development from concept to clinic. Let CamelBio be your reliable sourcing bridge for rare and routine targets alike.