Anti-ICAM5 (Telencephalin) Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q60625

Core Product Specifications and Parameters

Biological Background: ICAM5 (Telencephalin) Function and Localization

• ICAM5, also known as Telencephalin, is a cell adhesion molecule belonging to the immunoglobulin superfamily.
• It serves as a receptor ligand for the integrin ITGAL:ITGB2/LFA-1, facilitating neuron-leukocyte adhesion.
• ICAM5 mediates homophilic cell adhesion, which is essential for dendritogenesis and arborization of hippocampal neurons. Related references: PMID:22187327
• The protein is exclusively expressed in telencephalic regions of the brain.
• Subcellularly, it localizes to the cell membrane, enriching in dendritic spine membranes and the uropodium of cell projections.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, as indicated by database annotations.
• Structurally, it contains immunoglobulin-like domains and a transmembrane helix, typical of adhesion molecules.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 32–227), suggesting reactivity with surface-exposed epitopes; consider this for live-cell staining or surface protein detection assays.
• For Western blot, the predicted molecular weight is ~97 kDa; validate detection using mouse brain lysate or neuronal cell lines as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), optimize antigen retrieval and permeabilization protocols based on the sample type, given the membrane localization of ICAM5.
• ELISA applications may benefit from using recombinant ICAM5 protein as a standard; ensure appropriate blocking conditions to reduce non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to IVD raw materials, focusing on quality monoclonal antibodies like this ICAM5 clone for neuroscience and cell adhesion studies. Our supply capabilities include validated antibody pairs, optimized bulk reagents, and specialized sourcing for rare targets. Whether you need ancillary reagents or technical consulting from concept to clinic, CamelBio acts as your reliable procurement bridge in the IVD supply chain.