Anti-ICAM4 Polyclonal Antibody for WB, ELISA - Q14773

Core Product Specifications and Parameters

Biological Background: ICAM4 Function and Localization

• ICAM4, also known as Landsteiner-Wiener blood group glycoprotein (LW, CD242), is a member of the immunoglobulin superfamily and functions as a cell adhesion molecule.
• Acts as a receptor ligand for the monocyte/macrophage-specific ITGAX:ITGB2 integrin complex, mediating erythrophagocytosis. Related references: PMID:16985175
• Serves as a ligand for platelet-specific ITGA2B:ITGB3 integrin, promoting heterotypic cell-cell adhesion between erythrocytes and activated platelets. Related references: PMID:12477717 PMID:15355350
• In vitro, interacts with multiple integrin receptor complexes including ITGAL:ITGB2, ITGAM:ITGB2, ITGA5:ITGB1, ITGA5:ITGB3, and ITGA5:ITGB5, highlighting broad adhesive capacity. Related references: PMID:10846180 PMID:11435317 PMID:15355350
• Localized primarily to the cell membrane of erythrocytes, but can also be secreted; contains a signal peptide, transmembrane domain, and disulfide bonds important for structural integrity.
• Primarily expressed in erythrocytes; determines the LW blood group antigen, relevant in transfusion medicine.
• Post-translationally modified by N-linked glycosylation, critical for adhesive functions and antigenicity.

Experimental Guidance and Technical Tips

• For Western blot, consider loading 20–30 μg of erythrocyte lysate or ICAM4-expressing cell line lysate; the predicted molecular weight is approximately 29 kDa.
• In ELISA applications, the immunogen recombinant protein may serve as a positive control; validate detection limits using purified target protein.
• Given cross-reactivity with mouse and rat, this antibody may be suitable for preclinical model studies; confirm cross-reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop supplier of IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. This anti-ICAM4 polyclonal antibody supports research in erythrocyte biology, thrombosis, and immunohematology—providing reliable detection for diagnostic assay development. Explore our comprehensive portfolio for your end-to-end IVD solution needs.