Anti-ICAM3/CD50 Rabbit Monoclonal Antibody for WB, IHC-P, FC - P32942

Core Product Specifications and Parameters

Biological Background: ICAM3/CD50 Function and Localization

• ICAM3 (CD50) is a type I transmembrane glycoprotein of the immunoglobulin superfamily, also known as CDw50 and ICAM-R.
• ICAM3 functions as a cell adhesion molecule by serving as a ligand for the integrin LFA-1 (ITGAL/ITGB2), mediating leukocyte adhesion, signaling, and the phagocytosis of apoptotic neutrophils by macrophages. Related references: PMID:1448173 PMID:1448174 PMID:23775590
• It also acts as a ligand for the integrin ITGAD/ITGB2, expanding its role in immune cell interactions. Related references: PMID:8777714
• ICAM3 binds dendritic cell-specific ICAM-3-grabbing non-integrin (CD209) in a Ca2+-dependent manner, facilitating initial DC–T cell contacts, clustering, and T cell proliferation. Related references: PMID:10721994
• The protein is constitutively expressed on the surface of resting leukocytes but is absent from resting or cytokine-activated endothelial cells. Related references: PMID:8325327 PMID:1448174
• ICAM3 undergoes phosphorylation and glycosylation; its immunoglobulin-like domains are stabilized by disulfide bonds.
• The predicted molecular mass is approximately 59.5 kDa, with glycosylation resulting in an apparent weight of 60–85 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 30–114) of human ICAM3, encompassing the ligand-binding domain; this design may favor recognition of functionally relevant epitopes.
• For western blotting, validate detection in leukocyte lysates; expect a band around 60–85 kDa due to variable glycosylation.
• For immunohistochemistry, consider antigen retrieval and include appropriate positive controls such as tonsil or lymph node tissues.
• In flow cytometry, use live, non-permeabilized leukocytes to detect surface expression; titrate the antibody for optimal signal-to-noise.
• For immunofluorescence and immunocytochemistry, fix and permeabilize cells appropriately, and confirm localization at the plasma membrane.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for ICAM3/CD50-related immune assays, offering validated monoclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. From discovery to clinical applications, we provide reliable, scalable supply solutions to accelerate your diagnostic development.