Anti-ICAM2 / CD102 Rabbit Monoclonal Antibody for WB, ELISA - P35330

Core Product Specifications and Parameters

Biological Background: ICAM2 Function and Localization

• Intercellular adhesion molecule 2 (ICAM2), also known as CD102 or lymphocyte function-associated AG-1 counter-receptor, is a member of the immunoglobulin superfamily.
• ICAM2 serves as a ligand for the integrin LFA-1 (ITGAL:ITGB2), mediating leukocyte cell-cell adhesion on resting cells. Related references: PMID:1401904
• Additionally, ICAM2 functions as a receptor ligand for dendritic cell CD209, triggering transendothelial migration of dendritic cell precursors from blood into peripheral tissues and subsequently to lymphoid organs (By similarity).
• The protein is localized to the cell membrane and enriched in microvilli, consistent with its role in cell adhesion and immune surveillance.
• ICAM2 is expressed in endothelial cells and leukocytes, with high levels detected in the lung, highlighting its importance in pulmonary immune responses.
• ICAM2 is a transmembrane glycoprotein containing disulfide bonds and immunoglobulin-like domains in its extracellular region.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-240 of mouse ICAM2) covers the majority of the extracellular domain, so the antibody likely recognizes epitopes in this region and may be suitable for detecting surface-localized protein.
• For Western blot, a band at approximately 31 kDa is expected; however, glycosylation may cause a shift, so validate the observed molecular weight in the relevant sample system.
• Cross-reactivity with rat is reported; for ELISA, consider using mouse or rat ICAM2 recombinant protein or cell lysates, and include appropriate controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams streamlined access to validated rabbit monoclonal antibodies, including those targeting immune adhesion molecules like ICAM2. Beyond single reagents, we supply matched antibody pairs, optimized monoclonals, bulk ancillary reagents, and reliable sourcing of rare-target raw materials. With end-to-end support from concept to clinic, CamelBio is your trusted partner for IVD raw-material procurement and development.