Anti-ICAM1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P13597

Core Product Specifications and Parameters

Biological Background: ICAM1 Function and Localization

• ICAM1 (Intercellular adhesion molecule 1), also known as CD54 or MALA-2, is a cell surface glycoprotein belonging to the immunoglobulin superfamily.
• It serves as a ligand for lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1), mediating leukocyte adhesion and trans-endothelial migration.
• During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
• In epithelial cells, ICAM1 interaction with MUC1 facilitates cell aggregation.
• Subcellular localization is primarily at the cell membrane.
• Tissue expression is low on lymphocytes, macrophages, and endothelial cells but is strongly induced on fibroblasts and epithelial cells upon stimulation.
• Post-translational modifications include glycosylation, disulfide bond formation, and potential ubiquitination (Ubl conjugation).
• Alternative splicing generates multiple isoforms, which may exhibit tissue-specific functions.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning amino acids 120–320 of mouse ICAM1, corresponds to an extracellular region containing Ig-like domains; this design likely generates antibodies that recognize native ICAM1 across human, mouse, and rat due to sequence conservation.
• For Western blot (WB), a band around 59 kDa is expected; however, glycosylation may cause shifts, so validation with appropriate controls is recommended.
• In immunohistochemistry (IHC-P), consider antigen retrieval methods and test on tissues known to express ICAM1, such as inflamed endothelium.
• For ELISA, the antibody may function as a capture or detection reagent; pairing with other clones or formats may require optimization.
• Cross-reactivity with human and rat suggests suitability for multi-species studies, but confirm performance in the relevant sample system.
• Titrate the antibody to determine the optimal working concentration for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. For research on leukocyte adhesion, inflammation, and related pathways, this anti-ICAM1 polyclonal antibody supports robust detection across multiple species. Contact us to explore custom sourcing solutions for rare targets and full workflow support.