Anti-Icam1 (CD54) Polyclonal Antibody for WB, IF-P, ELISA - Q00238

Core Product Specifications and Parameters

Biological Background: Icam1 Function and Localization

• Intercellular adhesion molecule 1 (ICAM1), also known as CD54, is a cell surface glycoprotein that mediates cell-cell adhesion through interactions with integrins LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2).
• ICAM1 functions as a receptor ligand for LFA-1, providing a calibrated system that adjusts T-cell killing strength to antigen stimulation intensity.
• Through its interaction with MAC-1, ICAM1 facilitates adhesion between stimulated neutrophils and activated endothelial cells during inflammatory responses.
• During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups via ARHGEF26/SGEF and RHOG activation.
• ICAM1 also promotes cell aggregation in epithelial cells through direct interaction with MUC1.
• It is localized to the cell membrane and features a signal peptide, immunoglobulin domains, a transmembrane region, and potential N-glycosylation and ubiquitin-like conjugation sites.

Experimental Guidance and Technical Tips

• The immunogen covers the majority of the extracellular domain (aa 28-493), making this antibody suitable for detecting endogenous Icam1 in rat and mouse samples (confirmed cross-reactivity).
• For Western blotting, a band around 60 kDa is expected, but glycosylation may cause a shift; consider using deglycosylation enzymes if precise size confirmation is needed.
• For immunofluorescence (IF-P), validate fixation and permeabilization conditions in your specific cell or tissue type, as membrane-localized targets often require optimized protocols.
• In ELISA applications, this polyclonal antibody may serve as a capture or detection reagent; pairing with a monoclonal antibody could improve specificity in sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for manufacturers and research labs, offering a comprehensive portfolio that includes this anti-Icam1 polyclonal antibody for cell adhesion and inflammation studies. Our supply chain capabilities extend to validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing—positioning CamelBio as an efficient bridge from concept to clinical application.