Anti-ICAM-1/CD54 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 is a cell adhesion molecule that serves as a ligand for the integrin LFA-1 (ITGAL:ITGB2), mediating leukocyte adhesion and providing a calibrated system for T-cell killing. Related references: PMID:3086451 PMID:3340213 PMID:38195629
• It also functions as a receptor for MAC-1 (ITGAM:ITGB2), enabling adhesion between stimulated neutrophils and endothelial cells. Related references: PMID:1980124
• During leukocyte trans-endothelial migration, ICAM1 engagement triggers endothelial apical cup assembly via ARHGEF26/SGEF and RHOG. Related references: PMID:17875742
• In epithelial cells, ICAM-1 promotes cell aggregation through interaction with MUC1. Related references: PMID:11173916
• (Microbial infection) ICAM-1 acts as a receptor for major group rhinoviruses (A, B) and Coxsackievirus A21, facilitating viral entry.
• Upon HHV-8 infection, ICAM-1 is degraded by viral E3 ubiquitin ligase MIR2 to evade immune clearance.
• ICAM-1 is a single-pass type I membrane protein localized at the cell membrane; it is expressed on vascular endothelial cells, thymic epithelial cells, fibroblasts, and certain leukocytes, with low basal expression on peripheral blood leukocytes. Related references: PMID:3086451
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 28-480), suggesting that this polyclonal antibody may recognize surface-exposed epitopes; consider its potential for flow cytometry or live-cell staining with appropriate validation.
• For Western blotting, expect a band near the predicted molecular weight of ~58 kDa; however, glycosylation often results in a higher apparent molecular weight (75–100 kDa). Optimize reducing conditions and loading controls.
• For immunohistochemistry (paraffin sections), heat-induced antigen retrieval may improve detection of this membrane protein.
• In immunofluorescence/ICC, include a permeabilization step for intracellular detection or omit it to specifically visualize surface localization.
• The antibody cross-reacts with mouse and rat; validate performance in these species using relevant positive control tissues (e.g., inflamed endothelium) and appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—from concept to clinic. For ICAM-1/CD54 research and assay development, we offer validated antibody pairs, recombinant proteins, and optimized polyclonal antibodies like this one, alongside bulk ancillary reagents and custom sourcing for rare targets.