Anti-ICAM-1/CD54 Polyclonal Antibody for WB and ELISA - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54 or Major group rhinovirus receptor, is a cell adhesion molecule encoded by the ICAM1 gene (GeneID: 3383). It is a single-pass type I membrane protein belonging to the immunoglobulin superfamily.
• ICAM-1 serves as a ligand for the integrin LFA-1 (ITGAL:ITGB2), mediating leukocyte cell-cell adhesion and contributing to calibrated T-cell killing responses. Related references: PMID:3086451, PMID:3340213, PMID:38195629
• It also binds to MAC-1 (ITGAM:ITGB2) on stimulated neutrophils, promoting adhesion to endothelial cells. Related references: PMID:1980124
• During leukocyte trans-endothelial migration, ICAM-1 engagement triggers assembly of endothelial apical cups via ARHGEF26/SGEF and RHOG activation. Related references: PMID:17875742
• As a pathogen receptor, ICAM-1 acts as the entry receptor for major group rhinoviruses A and B and Coxsackievirus A21.
• Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, ICAM-1 is targeted for degradation by the viral E3 ubiquitin ligase MIR2, likely to evade immune detection.
• ICAM-1 is located on the cell membrane, with expression on non-hematopoietic cells (vascular endothelial cells, epithelial cells, fibroblasts) and hematopoietic cells (tissue macrophages, T-cell blasts, dendritic cells), and at low levels on peripheral blood leukocytes. Related references: PMID:3086451
• Key post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation. The protein contains immunoglobulin-like domains and a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of human ICAM-1 (amino acids 250-480), which may be suitable for detecting surface-expressed or soluble protein.
• For Western blotting, consider a predicted molecular weight of approximately 58 kDa; however, glycosylation and other modifications may shift the apparent molecular weight.
• Validate antibody reactivity in the relevant sample system, particularly when using mouse samples, due to cross-reactivity claims.
• For ELISA, test against recombinant ICAM-1 protein or cell lysates to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, labs, and research institutes to streamline IVD raw-material sourcing from discovery to commercialization. For ICAM-1/CD54 and related inflammatory or viral entry targets, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing of rare materials. Let CamelBio bridge your procurement needs with reliable, scalable supply solutions.