Anti-ICAM-1/CD54 Mouse Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P13597

Core Product Specifications and Parameters

Biological Background: Intercellular adhesion molecule 1 Function and Localization

• Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily that mediates cell-cell adhesion.
• As a ligand for the integrin receptors LFA-1 (ITGAL:ITGB2) and MAC-1 (ITGAM:ITGB2), ICAM-1 plays a critical role in leukocyte adhesion, enabling T-cell cytotoxicity and inflammatory responses.
• During leukocyte trans-endothelial migration, ICAM-1 facilitates the formation of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.
• In epithelial cells, ICAM-1 interacts with MUC1 to promote cell aggregation, highlighting its role in tissue organization beyond immune cells.
• ICAM-1 is expressed at low levels on subsets of lymphocytes, macrophages, and endothelial cells, but is strongly upregulated on these and on fibroblasts and epithelial cells upon activation.
• The protein contains multiple immunoglobulin-like domains, a transmembrane helix, and undergoes alternative splicing and post-translational modifications including glycosylation, disulfide bond formation, and ubiquitination.
• Subcellularly, ICAM-1 localizes predominantly to the cell membrane, consistent with its function as an adhesion receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 28-485) of mouse ICAM-1, ensuring recognition of the full-length membrane-bound form and potential soluble fragments.
• For Western blotting (WB), the predicted molecular weight is ~59 kDa; however, glycosylation may alter the apparent size. Validate band specificity using appropriate controls.
• For immunofluorescence (IF) and immunohistochemistry (IHC), optimize fixation and permeabilization conditions to detect membrane-localized ICAM-1. Paraffin-embedded sections may require antigen retrieval.
• Cross-reactivity with rat ICAM-1 allows for comparative studies, but confirm reactivity in the desired tissue or cell type.
• For ELISA, use recombinant or native ICAM-1 as reference and consider the influence of soluble ICAM-1 in biological fluids.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing this anti-ICAM-1/CD54 monoclonal antibody for cell adhesion and immunology research. Beyond individual antibodies, we offer matched antibody pairs, bulk reagents, and custom sourcing for rare targets—streamlining your development from concept to clinic. Whether you need ancillary raw materials or technical consulting, our supply chain is designed to meet diagnostic and laboratory demands efficiently.