Anti-ICAM-1/CD54 Monoclonal Antibody for WB, IHC-P, FC, ELISA - P05362

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 (Intercellular adhesion molecule 1), also known as CD54 or Major group rhinovirus receptor, functions as a cell adhesion molecule by binding to integrins LFA-1 and Mac-1, mediating leukocyte adhesion to endothelial cells and facilitating T-cell activation and trans-endothelial migration. Related references: PMID:3086451, PMID:3340213, PMID:38195629, PMID:1980124, PMID:17875742
• ICAM-1 promotes cell aggregation in epithelial cells through interaction with MUC1. Related references: PMID:11173916
• Acts as a receptor for major group rhinoviruses and coxsackievirus A21, facilitating viral entry into host cells.
• Subcellular localization is predominantly at the cell membrane.
• Constitutively expressed on vascular endothelial cells and inducible on various cell types, including epithelial cells, fibroblasts, and hematopoietic cells. Related references: PMID:3086451
• Undergoes N-linked glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, which regulate its function and stability.

Experimental Guidance and Technical Tips

• Immunogen: A synthetic peptide corresponding to the N-terminal region (amino acids 1–100) of human ICAM-1. Epitope accessibility may vary depending on fixation and denaturation conditions.
• For Western blotting (WB), expect a specific band around 58 kDa. Validate using positive controls such as HUVEC or activated PBMC lysates.
• For immunohistochemistry (IHC-P), antigen retrieval with citrate or EDTA buffer may be required; optimize primary antibody dilution and detection system.
• For flow cytometry (FC), use an appropriate antibody dilution and include Fc receptor blocking to minimize non-specific binding.
• For ELISA, coat antigen or capture antibody under optimized conditions; include standard curves for quantification.
• Store as recommended (cold shipment implying –20°C or 4°C storage); avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, including validated antibody pairs and optimized recombinant monoclonal antibodies. Our ICAM-1/CD54 rabbit monoclonal antibody supports research in cell adhesion, inflammation, and viral infection, and we offer bulk ancillary reagents and rare target sourcing to streamline your development pipeline. Contact us for integrated technical services and supply chain solutions from concept to clinic.