Anti-ICAM-1/CD54 Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P05362 / P13597

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 Function and Localization

• ICAM-1 is a cell adhesion molecule that serves as a receptor ligand for ITGAL:ITGB2/LFA-1 and ITGAM:ITGB2/MAC-1, mediating leukocyte cell-cell adhesion and calibrating T-cell killing strength, as well as adhesion between stimulated neutrophils and endothelial cells. Related references: PMID:3086451 PMID:3340213 PMID:38195629 PMID:1980124
• During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Related references: PMID:17875742
• ICAM-1 promotes cell aggregation in epithelial cells via interaction with MUC1. Related references: PMID:11173916
• Acts as a receptor for major group rhinoviruses and Coxsackievirus A21, and is targeted for degradation by Kaposi's sarcoma-associated herpesvirus to evade immune clearance.
• Subcellular localization: predominantly expressed on the cell membrane.
• Tissue specificity: expressed on non-hematopoietic cells such as vascular endothelial cells, thymic epithelial cells, fibroblasts, and on hematopoietic cells like tissue macrophages and mitogen-stimulated T lymphocyte blasts. Related references: PMID:3086451
• Post-translational modifications include glycosylation, disulfide bonds, phosphorylation, and ubiquitin-like conjugation; features immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant protein covering human and mouse ICAM-1; it is expected to recognize native and denatured forms in applications such as WB, IF/ICC, IF-P, IHC-P, and ELISA.
• For WB, a band size of approximately 58–59 kDa (human/mouse) should be observed; validate expression levels in the relevant sample system.
• In IF and IHC-P, a membrane-localized signal is anticipated; consider including appropriate controls to confirm specificity.
• Cross-reactivity with human, mouse, and rat makes this antibody suitable for translational studies across species; however, users are advised to verify performance in their specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our ICAM-1/CD54 antibody supports research into leukocyte adhesion, inflammation, and viral pathogenesis, enabling assay development in these areas. We offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD development and procurement needs.