Anti-ICAM-1/CD54 Monoclonal Antibody for Flow Cytometry - Q00238

Core Product Specifications and Parameters

Biological Background: ICAM-1/CD54 (Intercellular adhesion molecule 1) Function and Localization

• ICAM-1 is a cell surface glycoprotein and member of the immunoglobulin superfamily that serves as a ligand for the integrins LFA-1 (ITGAL:ITGB2) and Mac-1 (ITGAM:ITGB2), mediating leukocyte adhesion and signaling.
• In leukocyte trans-endothelial migration, ICAM-1 engagement triggers the assembly of endothelial apical cups via ARHGEF26/SGEF and RHOG activation, facilitating diapedesis.
• It promotes epithelial cell aggregation by interacting with MUC1, contributing to mucosal barrier integrity.
• The protein is a single-pass type I membrane protein with multiple immunoglobulin-like repeats, a transmembrane domain, and a cytoplasmic tail; it is localized to the cell membrane.
• Post-translational modifications include N-linked glycosylation and ubiquitination (Ubl conjugation), which may influence protein stability and interaction with adaptor proteins.
• As a key adhesion molecule, ICAM-1 is upregulated in response to inflammation and is involved in leukocyte recruitment, T-cell activation, and neutrophil adhesion.

Experimental Guidance and Technical Tips

• For flow cytometry with the conjugate, perform a titration using a relevant rat cell line or primary leukocytes to establish the optimal staining concentration, typically in the range of 0.1–1 µg per million cells.
• Since the immunogen covers the extracellular domain (aa 28–493), the antibody detects surface-expressed ICAM-1 without the need for permeabilization; confirm staining with a matched isotype control.
• Consider combining with cell viability dyes or other lineage markers for multi-parameter analysis of rat immune cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with high-quality IVD raw materials, including validated anti-ICAM-1/CD54 antibodies for immunology and inflammation research. We offer customized monoclonal and polyclonal antibody production, bulk ancillary reagents, and sourcing for rare targets, streamlining your development from discovery to clinical application.