Anti-IBTK Rabbit Polyclonal Antibody for WB and ELISA - Q9P2D0

Core Product Specifications and Parameters

Biological Background: IBTK Function and Localization

• IBTK (Inhibitor of Bruton tyrosine kinase), also known as BTKI or BTBD26, is a key regulator of B-cell receptor signaling.
• It functions as a negative regulator of BTK kinase activity, attenuating BTK-mediated calcium mobilization and NF-κB transcription.
• IBTK contains ANK repeats and has multiple isoforms due to alternative splicing; isoform 1 is the predominant form expressed across tissues.
• The protein localizes to the cytoplasm, membrane, and nucleus, suggesting dynamic shuttling or context-dependent roles.
• Highly expressed in hematopoietic tissues (fetal liver, spleen, lymph node, thymus, peripheral blood leukocytes, bone marrow) and lymphoid cell lines, supporting its role in B-cell development.
• Phosphorylation of IBTK has been identified by proteomic studies, indicating potential regulation by post-translational modification.
• As a reference proteome component, IBTK is evolutionarily conserved and essential for studies on B-cell malignancies and immunodeficiencies.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight of IBTK is 151 kDa; consider using a low-percentage gel and extended transfer times to ensure efficient transfer of this high-molecular-weight protein.
• In ELISA applications, the polyclonal nature of this antibody may provide robust signal amplification; consider direct or sandwich ELISA formats tailored to your assay requirements.
• Validate antibody performance in B-cell lines known to express high levels of IBTK (e.g., Jurkat, Ramos) to confirm specificity and sensitivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with validated raw materials for B-cell signaling targets like IBTK. From optimized polyclonal antibodies to ancillary reagents, our one-stop platform streamlines procurement for immunoassay development. Explore our portfolio for rare target antibodies and bulk supply solutions.