Anti-IAPP (Amylin, DAP) Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P10997

Core Product Specifications and Parameters

Biological Background: IAPP Function and Localization

• Islet amyloid polypeptide (IAPP), also known as amylin or diabetes-associated peptide (DAP), is a glucoregulatory peptide hormone with a molecular weight of approximately 9.8 kDa.
• IAPP selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in skeletal muscle, while sparing adipocyte glucose metabolism, thereby contributing to energy homeostasis. Related references: PMID:2690069
• Signaling occurs through calcitonin receptor (CALCR) and receptor activity-modifying protein (RAMP) complexes, forming high-affinity amylin receptors (AMYRs); IAPP can also bind CALCR alone with lower affinity (By similarity).
• The hormone is secreted from pancreatic islet β-cells and co-released with insulin in response to nutrient stimuli.
• Post-translational processing involves signal peptide removal, cleavage at paired basic residues to generate the 37-amino acid mature peptide, C-terminal amidation, and intrachain disulfide bond formation.
• IAPP is intrinsically amyloidogenic; its aggregation into amyloid deposits in pancreatic islets is a pathological hallmark of type 2 diabetes.
• UniProt keywords include: 3D-structure, Amidation, Amyloid, Cleavage on pair of basic residues, Disulfide bond, Hormone, Secreted, and Signal.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length human IAPP precursor (aa 1–89); the antibody may recognize both pro-IAPP and mature IAPP. Validate expected band sizes (~10 kDa for precursor, ~4 kDa for mature peptide) under reducing and non-reducing conditions.
• For Western blotting, a predominant band near 10 kDa is anticipated; use low molecular weight markers and optimize transfer conditions to retain small peptides.
• In immunohistochemistry (paraffin-embedded, IHC-P) and immunofluorescence (IF-P), employ antigen retrieval and include appropriate positive/negative controls, particularly in pancreatic or brain tissues where IAPP is expressed.
• ELISA applications can leverage the polyclonal's broad epitope coverage; consider pairing with a monoclonal capture antibody for sandwich assays to improve signal-to-noise ratio.
• Reported cross-reactivity with mouse and rat IAPP should be independently validated in the relevant sample system due to interspecies sequence differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with a comprehensive range of IVD raw materials, including validated polyclonal and monoclonal antibodies targeting metabolic and endocrine protein markers like IAPP. Our portfolio extends to antibody pairs for immunoassay development, bulk ancillary reagents, and customized sourcing solutions for rare or emerging targets. From assay prototyping to clinical application, CamelBio helps streamline your supply chain with one-stop convenience.