Anti-HUWE1 Polyclonal Antibody for WB and ELISA - Q7Z6Z7

Core Product Specifications and Parameters

Biological Background: HUWE1 Function and Localization

• HUWE1 is an E3 ubiquitin-protein ligase that targets proteins for proteasomal degradation via polyubiquitination, including MCL1, p53/TP53, core histones, MYCN, CDC6, NR1D1, and MFN2 (PubMed:15567145, PubMed:15767685, PubMed:15989957, PubMed:15989956, PubMed:18488021, PubMed:17567951, PubMed:20534529, PubMed:26214738). Related references: PMID:15567145 PMID:15767685 PMID:15989957 PMID:15989956 PMID:18488021 PMID:17567951 PMID:20534529 PMID:26214738
• It regulates apoptosis by polyubiquitinating and degrading MCL1, an anti-apoptotic member of the BCL2 family (PubMed:15989957). Related references: PMID:15989957
• HUWE1 monoubiquitinates DNA polymerase beta (POLB) at multiple lysines, implicating it in base-excision repair (PubMed:19713937). Related references: PMID:19713937
• It acts as a ubiquitin ligase for core histones (H1, H2A, H2B, H3, H4), linking it to chromatin regulation (PubMed:15567145, PubMed:15767685, PubMed:15989956). Related references: PMID:15567145 PMID:15767685 PMID:15989956
• HUWE1 localizes to the cytoplasm, nucleus, and mitochondrion, consistent with its diverse substrates and functions.
• It participates in regulating the JNK and NF-kappa-B pathways by assembling branched ubiquitin chains on substrates, protecting K63-linked chains from deubiquitination (PubMed:27746020). Related references: PMID:27746020
• The protein is expressed weakly in heart, brain, and placenta, and is predominantly found in colorectal carcinoma cell lines. It is implicated in neural differentiation, circadian rhythms, and mitochondrial dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1–100 of human HUWE1. When using rat samples, verify sequence homology in this region to ensure reactivity.
• For Western blot, due to the large molecular weight (~482kDa), consider using a low-percentage acrylamide gel (e.g., 6%) and extended transfer times to improve detection.
• ELISA can be employed for HUWE1 detection in cell or tissue lysates; validate the assay conditions in your specific experimental system.
• The antibody is supplied as a polyclonal rabbit IgG; pre-absorption or blocking peptides may be considered for specificity controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers seamless access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents—supporting your assay development for targets like HUWE1 in ubiquitination and oncology research. Whether you need rare-target sourcing or technical consulting, we help bridge your product pipeline from concept to clinic.