Anti-HUS1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60921

Core Product Specifications and Parameters

Biological Background: HUS1 Function and Localization

• HUS1 is a component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. Related references: PMID:21659603
• The 9-1-1 complex acts as a sliding clamp platform on DNA, stimulating DNA polymerase beta (POLB), flap endonuclease FEN1, and DNA ligase I (LIG1) to facilitate long-patch base excision repair (LP-BER). Related references: PMID:21659603
• The complex is necessary for the recruitment of RHNO1 to double-stranded breaks occurring during the S phase. Related references: PMID:21659603
• HUS1 localizes to the nucleus and cytoplasm, including the cytosol, and is ubiquitously expressed in human tissues.
• Keywords associated with HUS1 include 3D-structure, alternative splicing, DNA damage, nucleus, cytoplasm, proteomics identification, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human HUS1 protein (amino acids 1-280), suggesting the polyclonal antibody recognizes multiple epitopes. This may provide robust detection across applications.
• For Western blot (WB) analysis, the predicted molecular weight is approximately 32 kDa. Optimize protein loading, gel percentage, and transfer conditions to clearly resolve this band.
• When performing immunohistochemistry on paraffin-embedded sections (IHC-P), consider testing standard antigen retrieval protocols to enhance signal.
• This antibody is reported to cross-react with mouse and rat HUS1. Validate performance in your specific model system before large-scale experiments.
• As a polyclonal antibody, batch-to-batch variability may occur. Include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibodies and critical reagents. For DNA damage and cell cycle checkpoint research, we offer high-quality polyclonal antibodies like this anti-HUS1 antibody, alongside matching systems and bulk ancillary reagents to streamline your assay development. Whether you need customized antibody pairs or rare target sourcing, CamelBio supports your project from concept to clinic.