Anti-HUS1 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O60921

Core Product Specifications and Parameters

Biological Background: HUS1 Function and Localization

• HUS1 is a component of the heterotrimeric 9-1-1 checkpoint complex, which also includes RAD9 and RAD1, and is crucial for DNA repair and cell cycle checkpoint control.
  Related references: PMID:21659603
• The 9-1-1 complex is loaded onto DNA damage sites by the RAD17-replication factor C clamp loader, where it acts as a sliding clamp platform to recruit and stimulate DNA repair enzymes.
  Related references: PMID:21659603
• HUS1 enhances the activities of DNA polymerase beta (POLB), flap endonuclease FEN1, and DNA ligase I (LIG1) during long-patch base excision repair.
  Related references: PMID:21659603
• The 9-1-1 complex is required for the recruitment of RHNO1 to double-stranded breaks during S phase, indicating a role in replication-associated DNA damage response.
  Related references: PMID:21659603
• HUS1 localizes to both the nucleus and cytoplasm, consistent with its shuttling role in DNA damage signaling.
• It is ubiquitously expressed across tissues, reflecting its fundamental role in genome maintenance.
• The protein undergoes alternative splicing and is subject to proteomic identification, as noted in UniProt keywords.
• With a predicted molecular weight of ~32 kDa (observed at 32kDa), HUS1 is a conserved checkpoint protein.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 280 residues of human HUS1, enabling recognition of multiple epitopes; verify specificity for your isoform of interest.
• For western blotting, a strong band at ~32 kDa is expected; use appropriate loading controls and validate with known positive and negative lysates.
• In immunoprecipitation, confirm antibody-antigen complex formation and consider using low-salt lysis buffers to preserve protein interactions.
• For immunofluorescence/ICC, test fixation and permeabilization conditions to optimize nuclear and cytoplasmic staining.
• Cross-reactivity with mouse HUS1 allows direct translation to rodent model systems; confirm reactivity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. The HUS1 Rabbit pAb supports research into cell cycle checkpoints and DNA repair pathways. Let CamelBio streamline your supply chain from concept to clinic with dedicated technical and sourcing support.