Anti-HuR/ELAVL1 Rabbit Polyclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC, ELISA - Q15717

Core Product Specifications and Parameters

Biological Background: HuR/ELAVL1 Function and Localization

• HuR binds to AU-rich elements (AREs) and poly-U motifs in the 3'-UTR of target mRNAs, thereby increasing their stability and regulating their translation (PubMed:14517288, PubMed:18285462, PubMed:31358969, PubMed:14731398, PubMed:17632515).
  Related references: PMID:14517288, PMID:18285462, PMID:31358969, PMID:14731398, PMID:17632515
• In embryonic stem cells (ESCs), HuR preferentially binds and stabilizes mRNAs lacking N6-methyladenosine (m6A) modification, promoting ESC differentiation (By similarity).
• HuR is capable of binding m6A-containing mRNAs, such as MYC, contributing to MYC mRNA stability (PubMed:32245947).
  Related references: PMID:32245947
• Binds avidly to the AREs in FOS and IL3 mRNAs; recognizes a core AU-rich motif (AUUUA, AUUUUA, AUUUUUA) and a 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503).
  Related references: PMID:8626503
• Together with ZNF385A, binds the 3'-UTR of p53/TP53 mRNA, controlling its nuclear export and potentially modulating p53 expression (By similarity).
• Enhances leptin mRNA stability via its 3' UTR ARE (PubMed:29180010).
  Related references: PMID:29180010
• Subcellularly, HuR shuttles between nucleus, cytoplasm, stress granules, and P-bodies, linking its mRNA stabilization function to cellular compartmentalization.
• Ubiquitously expressed in tissues including brain, liver, thymus, and muscle. Post-translationally regulated by acetylation, phosphorylation, methylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen (aa 1-326 of human HuR) suggests the antibody may recognize multiple epitopes; validate performance in your specific experimental system.
• For Western blot, a major band at approximately 36kDa is expected. Use validated positive (e.g., HuR-overexpressing lysates) and negative (e.g., KO) controls for specificity.
• In IHC-P and IF/ICC, optimize antigen retrieval and permeabilization protocols; HuR's RNA-binding activity and subcellular localization may be affected by fixation.
• For ELISA, the polyclonal antibody can be used as capture or detection reagent; confirm suitable pairing with matched antibody pairs.
• Cross-reactivity with mouse and rat HuR is reported, facilitating translational research; independently verify reactivity in rodent samples.

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