Anti-Human/Mouse CD34 Rabbit PolymAb® Monoclonal Antibody for WB, IHC-P, ELISA - P28906 / Q64314

Core Product Specifications and Parameters

Biological Background: CD34 Function and Localization

• CD34, also known as hematopoietic progenitor cell antigen CD34, is a single-pass type I membrane glycoprotein encoded by the CD34 gene, with a calculated molecular weight of ~41 kDa.
• Acts as a possible adhesion molecule, mediating attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells.
• Functions as a scaffold for lineage-specific glycans, enabling stem cells to bind lectins on stromal cells or other marrow components.
• Presents carbohydrate ligands to selectins, facilitating cell-cell interactions.
• Selectively expressed on the surface of hematopoietic progenitor cells and on small vessel endothelium across various tissues.
• Undergoes alternative splicing, producing distinct isoforms that may modulate function.
• Subcellularly localized to the membrane, with a signal peptide and a single transmembrane helix.
• Post-translational modifications include N-linked glycosylation and phosphorylation, impacting protein stability and interactions.

Experimental Guidance and Technical Tips

• The immunogen is recombinant human CD34 protein; antibody reactivity with mouse CD34 is based on sequence homology; validate in your specific sample system.
• For Western blot, the expected band is at approximately 41 kDa; loading controls and optimized blocking/detection conditions are recommended.
• For immunohistochemistry (paraffin-embedded), consider antigen retrieval methods (e.g., heat-mediated citrate/EDTA buffer) and test several dilutions.
• For ELISA, this antibody may serve as detection or capture antibody; pairing with a different clone is suggested for sandwich assays.
• Cross-reactivity with human and mouse suggests suitability for studies in both species, but confirm in preliminary experiments.
• Since CD34 is expressed on endothelial cells and progenitor cells, ensure specificity in mixed cell populations via appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic development. Our CD34 Rabbit PolymAb® monoclonal antibody supports hematopoiesis, stem cell, and vascular biology research, with comprehensive supply capabilities to accelerate your project from concept to clinic. For rare target raw-material needs or custom solutions, rely on CamelBio's technical expertise and one-stop service.